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ClPLD5, a phospholipase gene is involved in protection of cytoskeleton stability in pollen of self-imcompatible ‘Xiangshui’ lemon ( Citrus limon )
Plant Cell, Tissue and Organ Culture ( IF 3 ) Pub Date : 2020-07-26 , DOI: 10.1007/s11240-020-01896-1
Wei Lin , Minhphu Do , Mi Wei , Xinhua He , Yuan Liu , Zhiyi Fan , Shuwei Zhang , Cong Luo , Guixiang Huang , Guibing Hu

Self-incompatibility (SI) is an important mechanism for the prevention of self-fertilization in plants and is one of the major causes of seedlessness in citrus plants. Phospholipase D (PLD) is closely related to the growth and development of pollen, but the role of PLD in citrus plants during SI is poorly understood. In this study, we identified PLDs associated with SI in citrus plants, analyzed 15 C2-PLDs encoding homologs of the C2 domain in the genomes of sweet orange (Citrus sinensis) and clementine mandarin (Citrus clementina). We performed a phylogenic analysis to classify the PLD subfamilies of homologous genes from other species and found that the C2-PLDs of ‘Xiangshui’ lemon shared high homology with C2-PLDs from pear and Arabidopsis. Expression analysis revealed that ClPLD5 is probably involved in the regulation of pollen of ‘Xiangshui’ lemon during SI. ClPLD5 is enriched in pollen and functions as a pollen protection factor, as overexpression of ClPLD5 in self-incompatible pollen prevented the depolymerization of the actin cytoskeleton during SI. This work provides insights into the regulation of the cytoskeleton in pollen under SI challenge in citrus plants, in which PLD plays a protective role in incompatible pollen.



中文翻译:

磷脂酶基因ClPLD5参与保护自交性“香水”柠檬(柑桔)花粉中的细胞骨架稳定性。

自交不亲和性(SI)是防止植物自我受精的重要机制,并且是柑桔类植物无核的主要原因之一。磷脂酶D(PLD)与花粉的生长和发育密切相关,但是人们对SI期间PLD在柑橘类植物中的作用知之甚少。在这项研究中,我们在柑橘类植物中鉴定了与SI相关的PLD,分析了15个编码甜橙(Citrus sinensis)和柑桔柑桔Citrus clementina)基因组C2域同源物的C2-PLD)。我们进行了系统发育分析,将其他物种的同源基因的PLD亚家族分类,发现“湘水”柠檬的C2-PLD与梨和拟南芥的C2-PLD具有高度同源性。表达分析表明,ClPLD5可能参与了SI期间“湘水”柠檬的花粉调节。ClPLD5富含花粉,并起花粉保护因子的作用,因为自交不亲和的花粉中ClPLD5的过度表达可防止SI期间肌动蛋白细胞骨架解聚。这项工作提供了对柑橘植物中SI挑战下花粉中细胞骨架调控的见解,其中PLD在不相容花粉中起保护作用。

更新日期:2020-07-26
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