BACKGROUND:The design and fabrication of hemocompatible and low-toxicity formulations remains a challenging task. Hydrogels are of considerable importance for biomedical applications since they are highly compatible with living tissue, both in vivo and in vitro. OBJECTIVE:The present study aimed todevelop and evaluate the characterizations and in vitro hemocompatibility of a hydrogel using polyvinyl alcohol and gelatin with different concentrations. METHODS:The gelling process was realized by cross-linking the polyvinyl alcohol and gelatin. The morphological and structural examinations of the synthetic hydrogels were done by scanning electron microscopy (SEM) and X-ray powder diffraction (XRD). The swelling behavior of the prepared hydrogels in water was evaluated. Prothrombin time, activated partial thromboplastin time, and thrombin time were measured, and a hemolysis test was done to evaluate the hemocompatibility of prepared hydrogels. RESULTS:The increase of the gelatin concentration in polyvinyl gelatin hydrogel increases the porosity and enhances the absorptivity of the prepared hydrogel. The measured hematological parameters indicated enhancement of hemocompatibility as the gelatin concentration was increased in the prepared hydrogel. CONCLUSIONS:The results obtained from this study confirm that gelatin was able to improve the properties of the polyvinyl alcohol–gelatin hydrogel and enhance the hemocompatibility. Thus, the prepared hydrogel could be used in a variety of biomedical applications.

中文翻译：

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明胶浓度对聚乙烯醇-明胶水凝胶的特性和血液相容性的影响。

背景：血液相容性和低毒性制剂的设计和制造仍然是一项具有挑战性的任务。水凝胶在生物医学应用中具有相当重要的意义，因为它们在体内和体外都与活组织高度相容。目的：本研究旨在开发和评估使用不同浓度聚乙烯醇和明胶的水凝胶的特性和体外血液相容性。方法：通过聚乙烯醇和明胶的交联实现胶凝过程。通过扫描电子显微镜 (SEM) 和 X 射线粉末衍射 (XRD) 对合成水凝胶的形态和结构进行了检测。评价制备的水凝胶在水中的溶胀行为。凝血酶原时间、活化部分凝血活酶时间、测定凝血酶时间，并进行溶血试验以评价制备的水凝胶的血液相容性。结果：聚乙烯明胶水凝胶中明胶浓度的增加使制备的水凝胶的孔隙率增加，吸收率提高。测量的血液学参数表明，随着制备的水凝胶中明胶浓度的增加，血液相容性增强。结论：本研究的结果证实，明胶能够改善聚乙烯醇-明胶水凝胶的性能并提高血液相容性。因此，制备的水凝胶可用于各种生物医学应用。聚乙烯基明胶水凝胶中明胶浓度的增加增加了孔隙率并增强了制备的水凝胶的吸收率。测量的血液学参数表明，随着制备的水凝胶中明胶浓度的增加，血液相容性增强。结论：本研究的结果证实，明胶能够改善聚乙烯醇-明胶水凝胶的性能并提高血液相容性。因此，制备的水凝胶可用于各种生物医学应用。聚乙烯基明胶水凝胶中明胶浓度的增加增加了孔隙率并增强了制备的水凝胶的吸收率。测量的血液学参数表明，随着制备的水凝胶中明胶浓度的增加，血液相容性增强。结论：本研究的结果证实，明胶能够改善聚乙烯醇-明胶水凝胶的性能并提高血液相容性。因此，制备的水凝胶可用于各种生物医学应用。从这项研究中获得的结果证实，明胶能够改善聚乙烯醇-明胶水凝胶的性能并提高血液相容性。因此，制备的水凝胶可用于各种生物医学应用。从这项研究中获得的结果证实，明胶能够改善聚乙烯醇-明胶水凝胶的性能并提高血液相容性。因此，制备的水凝胶可用于各种生物医学应用。