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Generating mucosal and systemic immune response following vaccination of vibrio cholerae adhesion molecule against shigella flexneri infection.
Indian Journal of Medical Microbiology ( IF 1.6 ) Pub Date : 2020-01-01 , DOI: 10.4103/ijmm.ijmm_19_411
Sumarno Reto Prawiro 1 , Sri Poeranto 2 , Aisyah Amalia 3 , Elsa Larissa Widyani 3 , Genitri Indraswari 3 , Merika Soraya 3 , Septha Rully Dwi Pradipto 3 , Adrian Prasetya 3 , Guntur Rizal Hidayat 4 , Safira Nur Alitha Putri 4
Affiliation  


Introduction: Previous studies have shown 37.8 kDa pili subunit protein of Vibrio cholerae and 49.8 kDa pili subunit protein of Shigella flexneri can act as an adhesion molecule to initiate infection. These molecules also have the ability to agglutinate blood. The present study assessed mucosal and systemic immunity following vaccination using 37.8 kDa V. cholerae and protection against S. flexneri. Subjects and Methods: Haemagglutination test was performed after purification of V. cholerae protein, followed by an anti-haemagglutination test. The intestinal weight and colony count were used to validate the protective effect on balb/c mice which were divided into the naive group, Shigella-positive control group, Vibrio-positive control group, V. cholerae infected-Vibrio-vaccinated group and S. flexneri-infected-Vibrio-vaccinated group. Th17, Treg, interleukin (IL) IL-17A, β-defensin and secretory-immunoglobulin A (s-IgA) were also measured to determine the systemic and mucosal immunity after vaccination. Results: The haemagglutination and anti-haemagglutination tests showed that the 37.8 kDa protein could inhibit 49.8 kDa of the S. flexneri pili subunit. Decreased intestinal weight and colony count of vaccinated group compared to naive group also support cross reaction findings. Vaccination also generates higher level of Th17, Treg, IL-17A, β-defensin and s-IgA significantly. Conclusions: 37.8 kDa subunit pili can act as a homologous vaccine candidate to prevent V. cholerae and S. flexneri infection.


中文翻译:

接种霍乱弧菌粘附分子以抵抗弗氏志贺氏菌感染后,产生粘膜和全身免疫反应。


简介:先前的研究表明霍乱弧菌的37.8 kDa菌毛蛋白亚基蛋白和弗氏志贺氏菌的49.8 kDa菌毛蛋白亚基可以作为黏附分子引发感染。这些分子也具有凝集血液的能力。本研究评估了以下使用37.8 kDa的疫苗的粘膜和系统免疫霍乱弧菌和防止弗氏志贺菌对象和方法:霍乱弧菌纯化后进行血凝试验蛋白质,然后进行抗血凝试验。肠道重量和菌落数用于验证对balb / c小鼠的保护作用,将其分为幼稚组,志贺菌阳性对照组,弧菌阳性对照组,霍乱弧菌感染-弧菌疫苗接种组和S。弗氏感染的弧菌疫苗接种组。还测量了Th17,Treg,白介素(IL)IL-17A,β-防御素和分泌型免疫球蛋白A(s-IgA),以确定接种疫苗后的全身和粘膜免疫力。结果:血凝和抗血凝试验表明37.8 kDa的蛋白可抑制49.8 kDa的肝细胞癌。弗氏链球菌菌毛亚单位。与未接种组相比,接种组肠道重量和菌落数减少也支持交叉反应发现。疫苗接种还会产生更高水平的Th17,Treg,IL-17A,β-防御素和s-IgA。结论: 37.8 kDa亚单位菌毛可作为预防霍乱弧菌弗氏链球菌感染的同源疫苗。
更新日期:2020-01-01
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