Transcriptional dynamics of cancer cells govern cell fate decisions and are therapeutically actionable drug targets. In this study, we engineered a circulating cancer cell line that secretes a luciferase reporter to capture constitutive and circadian clock‐driven transcription dynamics over the course of a day. Engineered human leukemic T cells (Jurkat) were observed to rhythmically secrete luciferase in a continuous flow cell culture system. When transplanted in vivo, engineered leukemic cells caused circadian plasma luciferase activity and had expected pathological signs of leukemic disease. This technique is rapid and noninvasive, requiring only a few microliters of media or blood, and can aid in investigating relationships between in vivo cancer cell signaling and behavior, such as diet or sleep.

中文翻译：

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使用基于血液的报告基因检测跟踪体内白血病 T 细胞转录动力学。

癌细胞的转录动力学控制着细胞命运的决定，并且是治疗上可行的药物靶点。在这项研究中，我们设计了一种循环癌细胞系，该细胞系分泌荧光素酶报告基因，以在一天内捕获组成型和生物钟驱动的转录动态。在连续流动细胞培养系统中观察到工程改造的人类白血病 T 细胞 (Jurkat) 有节奏地分泌荧光素酶。在体内移植时，工程白血病细胞会引起昼夜节律血浆荧光素酶活性，并具有预期的白血病病理学迹象。这种技术快速且无创，只需要几微升的培养基或血液，可以帮助研究体内 癌细胞信号和行为，例如饮食或睡眠。