Reproductive Toxicology ( IF 3.3 ) Pub Date : 2020-07-25 , DOI: 10.1016/j.reprotox.2020.07.009 Paweł Radwan 1 , Bartosz Wielgomas 2 , Michał Radwan 3 , Rafał Krasiński 4 , Anna Klimowska 2 , Dorota Kaleta 5 , Joanna Jurewicz 5
Bisphenol A (BPA) is a widespread environmental endocrine disrupting chemical. Although many animals and in vitro studies reported that BPA may affect female fertility through the effect on maturing oocytes and meiotic cell division, but the data from human studies are limited and inconclusive. The study was conducted to examine the association between urinary BPA concentration and in vitro reproductive outcomes (metaphase II (MII) oocyte yield, top quality embryo, fertilization rate, implantation rate and clinical pregnancy) among women from an infertility clinic.
The study participants were enrolled in the Infertility Center in Poland. 450 women aged 24-44 (n = 674 IVF cycles) provided urine samples. The urinary concentrations of BPA were evaluated using validated gas chromatography ion-tap mass spectrometry method. Clinical outcomes of IVF treatment were abstracted from patients electronic chart records. To assess the relationship between urinary BPA concentrations early examined reproductive outcomes generalized linear mixed models were used.
The detection rate of BPA in urine samples was 98% and the geometric mean 1.59 ± 2.15 ng/ml. A significant decrease was observed between urinary concentration of BPA and implantation (p = 0.04) and decreased MII oocyte count (p = 0.03). There was no association between other examined IVF outcomes: embryo quality, fertilization rate and clinical pregnancy and BPA exposure.
Exposure to BPA may have a negative effect during the early stages of human development. The studies among the larger and more diverse population are needed to confirm the results.
中文翻译:
来自生育诊所的女性的尿双酚 A 浓度和体外受精结果。
双酚 A (BPA) 是一种广泛存在的环境内分泌干扰化学品。尽管许多动物和体外研究报告称,BPA 可能通过对成熟卵母细胞和减数分裂细胞分裂的影响来影响女性生育能力,但来自人类研究的数据有限且无定论。该研究旨在检查来自不孕症诊所的女性尿 BPA 浓度与体外生殖结果(中期 II (MII) 卵母细胞产量、优质胚胎、受精率、植入率和临床妊娠)之间的关系。
研究参与者被登记在波兰的不孕症中心。450 名 24-44 岁的女性(n = 674 个体外受精周期)提供了尿液样本。使用经过验证的气相色谱离子抽头质谱法评估 BPA 的尿液浓度。IVF 治疗的临床结果是从患者电子图表记录中提取的。为了评估尿 BPA 浓度之间的关系,使用了早期检查的生殖结果广义线性混合模型。
尿样中BPA的检出率为98%,几何平均值为1.59±2.15 ng/ml。在 BPA 的尿浓度和植入之间观察到显着降低 (p = 0.04) 和 MII 卵母细胞计数减少 (p = 0.03)。其他检查的 IVF 结果之间没有关联:胚胎质量、受精率和临床妊娠和 BPA 暴露。
在人类发育的早期阶段,接触 BPA 可能会产生负面影响。需要在更大和更多样化的人群中进行研究以确认结果。