Plant Communications ( IF 10.5 ) Pub Date : 2020-07-25 , DOI: 10.1016/j.xplc.2020.100102 Florian Veillet 1, 2, 3 , Mickael Durand 4 , Thomas Kroj 3 , Stella Cesari 3 , Jean-Luc Gallois 5
Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes, the CRISPR technology has revolutionized plant research and precision crop breeding. The CRISPR toolbox holds great promise in the production of crops with genetic disease resistance to increase agriculture resilience and reduce chemical crop protection with a strong impact on the environment and public health. In this review, we provide an extensive overview on recent breakthroughs in CRISPR technology, including the newly developed prime editing system that allows precision gene editing in plants. We present how each CRISPR tool can be selected for optimal use in accordance with its specific strengths and limitations, and illustrate how the CRISPR toolbox can foster the development of genetically pathogen-resistant crops for sustainable agriculture.
中文翻译:
用 CRISPR 实现精准育种:通过对病原体的遗传抗性来说明
自从发现细菌适应性免疫系统并开发真核生物基因组编辑以来,CRISPR 技术彻底改变了植物研究和精准作物育种。CRISPR 工具箱在生产具有遗传抗病性的作物方面具有广阔的前景,可提高农业恢复力并减少对环境和公共健康产生重大影响的化学作物保护。在这篇综述中,我们广泛概述了 CRISPR 技术的最新突破,包括新开发的可在植物中进行精确基因编辑的主要编辑系统。我们介绍了如何根据其特定的优势和局限性选择每个 CRISPR 工具以实现最佳使用,