The metabolites present in Opuntia species have been widely studied by their health-promoting effects, but there is no information about the compounds present in Opuntia callus or their biological effects. The aim of this study was to identify the metabolites in Opuntia ficus indica, Opuntia megacantha and Opuntia streptacantha callus ethanolic extracts, and to analyze their antioxidant, anti-inflammatory and anti-adipogenic properties in mammalian cells. The compounds identified by chromatographic analysis and mass spectroscopy showed that the Opuntia callus from the three species synthesized 3,5-Di-O-galloylshikimic acid as major compound, as well as gallic acid and epicathequin derivates in lower concentration. The calluses metabolites reduced the formation of reactive oxygen species in Caco-2 cells similarly to the flavonoid isorhamnetin. The anti-inflammatory activity, measured as the production of nitric oxide, was inhibited between 20 and 68% in a murine macrophage cell line. This inhibition was higher with O. streptacantha callus extracts, followed by those of O. megacantha and O. ficus-indica. Additionally, a reduction on the lipid accumulation in 3T3 cells was observed, during and after adipocyte differentiation using extracts from the three Opuntia species. Our results prove that Opuntia calluses are a source of compounds with pharmacological relevance.

仙人掌种类中存在的代谢产物已经通过促进健康的作用得到了广泛研究，但是尚无关于仙人掌愈伤组织中存在的化合物或其生物学作用的信息。这项研究的目的是鉴定印度仙人掌，大仙人掌和链霉菌愈伤组织乙醇提取物中的代谢物，并分析它们在哺乳动物细胞中的抗氧化，抗炎和抗脂肪形成特性。通过色谱分析和质谱鉴定的化合物表明，仙人掌这三个物种的愈伤组织合成了3,5-二-O-galloylshikimicmic酸作为主要化合物，以及没食子酸和艾奇霉素的浓度较低。与类黄酮异鼠李素类似，老茧代谢产物减少了Caco-2细胞中活性氧的形成。以一氧化氮的产生来衡量的抗炎活性在鼠巨噬细胞系中被抑制在20％至68％之间。O. streptacantha愈伤组织提取物的抑制作用更高，其次是O. megacantha和O. ficus-indica。此外，使用三种仙人掌提取物在脂肪细胞分化期间和之后观察到3T3细胞脂质积累的减少。种类。我们的结果证明，仙人掌愈伤组织是具有药理学意义的化合物的来源。