α-l-Arabinofuranosidase (PsGH43_12) of family 43 glycoside hydrolase and subfamily 12 from Pseudopedobacter saltans was cloned, over-expressed and biochemically characterized. PsGH43_12 displayed molecular mass, ~ 65 kDa. It exhibited activity in pH (5–9) and temperature range (35–55 °C) with maxima at pH 6.5 and 50 °C. PsGH43_12 gave 88.7 U/mg specific activity against rye arabinoxylan and 78.9 U/mg against wheat arabinoxylan. PsGH43_12 displayed K_m and V_max, 3.02 mg/ml and 103 µmole/min/mg, respectively, against rye arabinoxylan and 2.17 mM and 100.7 µmole/min/mg, respectively, against pNP-α-l-arabinofuranoside. 10 mM Mg²⁺ or Ca²⁺ ions enhanced PsGH43_12 activity by 54% or 33%, respectively. PsGH43_12 hydrolyzed rye arabinoxylan and released only l-arabinosyl moiety as main product, confirming its specificity towards α-l-arabinofuranoside. The regioselective analysis by NMR showed that PsGH43_12 belongs to type III α-l-arabinofuranoside. The synergistic behavior of PsGH43_12 in saccharification of mild alkali pretreated finger miller stalk (FMS) along with xylanase (CtXyn11A) from Clostridium thermocellum and xylosidase (BoGH43) from Bacteroides ovatus gave twofold higher total reducing sugar (TRS) yield. TLC analysis of pretreated FMS hydrolysed by CtXyn11A and BoGH43 showed xylooligosaccharides and xylose. Addition of PsGH43_12 to above combination gave mostly xylose and arabinose confirming their synergistic behavior and displaying their applicability in hydrolysis of hemicellulosic biomass.

克隆，过表达和生化鉴定了43个糖苷水解酶的家族的α- 1-阿拉伯呋喃糖苷酶（Ps GH43_12）和来自盐假拟杆菌的亚家族12 。Ps GH43_12的分子量约为65 kDa。它在pH（5–9）和温度范围（35–55°C）中表现出活性，在pH 6.5和50°C时达到最大值。Ps GH43_12对黑麦阿拉伯木聚糖的比活性为88.7 U / mg，对小麦阿拉伯木聚糖的比活性为78.9 U / mg。Ps GH43_12对黑麦阿拉伯木聚糖的K _m和V _max分别为3.02 mg / ml和103 µmole / min / mg，对p分别为2.17 mM和100.7 µmole / min / mg。NP-α- 1-阿拉伯呋喃糖苷。10 mM Mg ²⁺或Ca ²⁺离子分别提高Ps GH43_12活性54％或33％。Ps GH43_12水解黑麦阿拉伯木聚糖并仅释放1-阿拉伯糖基部分作为主要产物，证实了其对α- 1-阿拉伯呋喃糖苷的特异性。NMR的区域选择性分析显示，Ps GH43_12属于III型α- 1-呋喃糖呋喃糖苷。的协同行为诗GH43_12在温和碱预处理的手指米勒茎（FMS）的糖化用木聚糖酶一起（的Ct从Xyn11A）热纤梭菌卵形拟杆菌和木糖苷酶（Bo GH43）的总还原糖（TRS）产量提高了两倍。Ct Xyn11A和Bo GH43水解的预处理FMS的TLC分析显示木寡糖和木糖。将Ps GH43_12添加到上述组合中，主要得到木糖和阿拉伯糖，证实了它们的协同行为并显示了它们在半纤维素生物质水解中的适用性。