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Characterization of Invertase and Diastase Activities, 5-Hydroxymethylfurfural Content and Hydrogen Peroxide Production of Some Algerian Honeys
Iranian Journal of Science and Technology, Transactions A: Science ( IF 1.7 ) Pub Date : 2020-07-25 , DOI: 10.1007/s40995-020-00936-x
Chahra Makhloufi , Khaled Taïbi , Leila Ait Abderrahim

Sixty-six honey samples collected from various regions in Algeria were explored for their enzymes activities, hydrogen peroxide production, and 5-hydroxymethylfurfural (HMF) content in the aim to enrich national and international database information about the quality of Algerian honeys. Correlations among the studied parameters were also investigated. Regarding the botanical origins of the selected honeys, 7 samples belong to Hedysarum coronarium, 18 to Eucalyptus spp., 6 to Pimpinella anisum, 1 to Cistus sp., 1 to Tamarix sp., 2 to Rubus sp., 1 to Daucus sp., 1 to Vicia sp., 2 to Citrus sp., 1 to Centaurea sp., 1 to Sedum sp. and 25 samples are multifloral. The obtained results demonstrated that invertase and diastase activities, HMF content and hydrogen peroxide accumulation vary significantly among samples even within the same honey type. In general, the diastatic activity ranged from 4 to 40 DN while invertase activity varied from 0 to 20 IN. The relation between both enzymes, expressed by the invertase/diastase ratio, exhibited index values ranging from 0 to 1.45 for all samples. Besides, HMF content ranged between 0.5 and 52.8 mg/kg and the hydrogen peroxide accumulation fluctuated from 0 to 30 µg/g/h. Significant positive correlation was established between invertase and diastase activities (r value = 0.47**), whereas negative correlations were revealed between diastatic activity and HMF content (r value = − 0.32*) from apart, and between invertase activity and HMF content from another part (r value = − 0.47**). It seems that invertase activity is a more sensitive indicator of heating, conditioning and storing time than the other studied parameters. However, HMF content and hydrogen peroxide offer a fast and good indication of honey quality.



中文翻译:

某些阿尔及利亚蜂蜜的转化酶和扩张酶活性,5-羟甲基糠醛含量和过氧化氢生成的表征

对从阿尔及利亚各个地区收集的66份蜂蜜样品进行了酶活性,过氧化氢生成和5-羟甲基糠醛(HMF)含量的研究,以丰富有关阿尔及利亚蜂蜜质量的国家和国际数据库信息。还研究了研究参数之间的相关性。关于选定蜂蜜的植物来源,有7个样品属于Hedysarum coronarium,18个样品属于桉树属,6个样品属于茴香皮,1个属于Cistus sp。,1个属于Tamarix sp。,2个属于Rubus sp。,1个属于Daucus sp。 ,1至Vicia sp。,2至Citrus sp。,1至矢车菊属,1至景天属。25个样品是多花的。获得的结果表明,即使在相同的蜂蜜类型中,样品之间的转化酶和淀粉酶活性,HMF含量和过氧化氢积累也存在显着差异。通常,透尿活性为4至40DN,而转化酶活性为0至20IN。对于所有样品,两种酶之间的关系均以转化酶/淀粉酶比率表示,其指数值范围为0至1.45。此外,HMF的含量在0.5至52.8 mg / kg之间,过氧化氢的积累在0至30 µg / g / h之间波动。在转化酶和淀粉酶活性之间建立了显着的正相关(r值= 0.47 **),而在其他方面,则显示出透析活性与HMF含量之间的负相关(r值=-0.32 *),而另一部位的转化酶活性与HMF含量之间存在负相关(r值=-0.47 **)。似乎转化酶活性比其他研究参数更敏感地指示加热,调节和储存时间。但是,HMF含量和过氧化氢可以快速,很好地表明蜂蜜的品质。

更新日期:2020-07-25
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