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Biochemical and Molecular Characterization of Lactic Acid Bacteria (LAB) Isolated from Fermented Pulses
Iranian Journal of Science and Technology, Transactions A: Science ( IF 1.7 ) Pub Date : 2020-07-25 , DOI: 10.1007/s40995-020-00934-z
Hiren A. Dhameliya , Sureshkumar N. Mesara , Himanshu Mali , Chandni Shah , Ramalingam Bagavathi Subramanian

Pulses are excellent source of protein, fiber and other key nutrients. Fermented pulse-based products are routinely used in many parts of India. In this study, an attempt has been made to isolate potent probiotic lactic acid bacteria from traditionally fermented Vigna radiata (mung beans) and Cajanus cajan (pigeon pea). Fermented seed samples of both the pulses were used for the isolation of lactic acid bacteria using Lactobacilli selection medium de Man, Rogosa and Sharpe. Twenty colonies were randomly selected, based on differential colony morphology. Only Gram-positive and catalase-negative isolates were further propagated for biochemical characterization. The isolates obtained showed different growth patterns at acidic conditions (pH 2, 3 and 4) and at high bile salt concentration (0.5% and 1.0%). Most of the isolates showed promising results in antibiotic resistance assay and cell surface hydrophobicity test. Three potent isolates were identified as Pediococcus pentosaceus (RBS1), Enterococcus durans (RBS3) and Pediococcus pentosaceus (RBS10) using 16S rDNA sequencing.



中文翻译:

发酵脉冲分离的乳酸菌(LAB)的生化和分子表征

豆类是蛋白质,纤维和其他关键营养素的极好来源。印度许多地方通常使用基于发酵脉冲的产品。在这项研究中,已经尝试从传统发酵的Vigna radiata(绿豆)和Cajanus cajan(鸽子豌豆)中分离有效的益生菌乳酸菌。将两个脉冲的发酵种子样品用于使用乳杆菌分离乳酸菌选择中等德曼,罗戈萨和夏普。根据不同的菌落形态随机选择20个菌落。仅将革兰氏阳性和过氧化氢酶阴性的分离物进一步繁殖以进行生化鉴定。获得的分离物在酸性条件(pH 2、3和4)和高胆汁盐浓度(0.5%和1.0%)下显示出不同的生长模式。大多数分离物在抗生素抗性测定和细胞表面疏水性测试中显示出令人鼓舞的结果。使用16S rDNA测序鉴定了三个有效分离株:戊糖戊球菌(RBS1),杜伦肠球菌(RBS3)和戊糖球菌(RBS10)。

更新日期:2020-07-25
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