Background

Phospholipase C (PLC) is an enzyme that hydrolyzes phospholipids and plays an important role in plant growth and development. The Brachypodium distachyon is a model plant of Gramineae, but the research on PLC gene family of Brachypodium has not been reported.

Objective

This study was performed to identify the PLC family gene in Brachypodium and to determine the expression profiles of PLCs under the abiotic stress.

Methods

Complete genome sequences and transcriptomes of Brachypodium were downloaded from the PLAZA. The hidden Markov model-based profile of the conserved PLC domain was submitted as a query to identify all potential PLC domain sequences with HMMER software. Expression profiles of BdPLCs were obtained based on the qRT-PCR analysis.

Results

There were 8 PLC genes in Brachypodium (BdPI-PLCs 1–4 and BdNPCs 1–4). All members of BdPI-PLC had three conserved domains of X, Y, and C2, and no EF-hand was found. All BdNPCs contained a phosphatase domain. BdPI-PLC genes were distributed on Chr1, Chr2 and Chr4, with different types and numbers of cis-regulatory elements in their respective gene promoters. Phylogenetic analysis showed that the genetic relationship between Brachypodium and rice was closer than Arabidopsis. The expression patterns of BdPI-PLC gene under abiotic stresses (drought, low temperature, high temperature and salt stress) were up-regulated, indicated their important roles in response to low temperature, high temperature, drought and salt stresses.

Conclusions

This study provides comprehensive information for the study of Brachypodium PLC gene family and lays a foundation for further research on the molecular mechanism of Brachypodium stress adaptation.

中文翻译：

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磷脂酶C基因家族在短枝曲霉中的表达和进化。

背景

磷脂酶C（PLC）是一种水解磷脂的酶，在植物的生长发育中起着重要的作用。在穗短柄草是禾本科的模式植物，但对PLC基因家族短柄的研究尚未见报道。

目的

进行了这项研究，以鉴定短臂猿中的PLC家族基因，并确定非生物胁迫下PLC的表达谱。

方法

完整的基因组序列和转录组的转录组是从PLAZA下载的。提交了保守的PLC域的基于隐式马尔可夫模型的概要文件作为查询，以使用HMMER软件识别所有潜在的PLC域序列。基于qRT-PCR分析获得了BdPLC的表达谱。

结果

腕足动物中有8个PLC基因（BdPI-PLCs 1-4和BdNPCs 1-4）。BdPI-PLC的所有成员均具有X，Y和C2的三个保守域，未发现EF-hand。所有BdNPC都包含一个磷酸酶结构域。BdPI-PLC基因分布在Chr1，Chr2和Chr4上，在各自的基因启动子中具有不同类型和数量的顺式调控元件。亲缘关系分析表明，水曲霉与水稻的遗传关系比拟南芥更近。BdPI-PLC的表达模式 非生物胁迫（干旱，低温，高温和盐胁迫）下的基因上调，表明它们在应对低温，高温，干旱和盐胁迫下的重要作用。

结论

本研究为腕足动植物PLC基因家族的研究提供了全面的信息，为进一步开展腕足动植物应激适应的分子机制奠定了基础。