当前位置: X-MOL 学术J. Innov. Opt. Health Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A novel fluorogenic probe for visualizing the hydrogen peroxide in Parkinson’s disease models
Journal of Innovative Optical Health Sciences ( IF 2.5 ) Pub Date : 2020-04-30 , DOI: 10.1142/s1793545820500133
Gaobin Zhang 1 , Zheng Li 2 , Fangjie Chen 1 , Duoteng Zhang 2 , Wenhui Ji 2 , Zhengpeng Yang 1 , Qiong Wu 2 , Chengwu Zhang 2 , Lin Li 2 , Wei Huang 2, 3
Affiliation  

Parkinson’s disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H2O2) in organisms. Developing an efficient method for noninvasive and real-time H2O2 detection is beneficial to investigate the role played by H2O2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H2O2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H2O2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H2O2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H2O2 detection, it has been successfully applied to visualizing the H2O2 concentration in living cells, Zebrafish and [Formula: see text]. elegans PD models.

中文翻译:

一种用于在帕金森病模型中可视化过氧化氢的新型荧光探针

帕金森病(PD)与过量过氧化氢(H22) 在有机体中。开发一种有效的无创和实时 H 方法22检测有利于调查H所起的作用22在 PD。在这项工作中,一种用于活生物体 H 的新型荧光探针 (CBH)22检测已经被设计、合成和表征。PBS溶液中CBH的发射非常微弱。然而,当 H22加入后,CBH溶液的荧光急剧增加12倍,同时发射峰从600 nm蓝移到530 nm。此外,CBH 对 H 的响应22具有高灵敏度和选择性,不受各种ROS/RNS、阴离子、阳离子和氨基酸的影响。基于 CBH 对 H 的良好性能22检测,它已成功应用于可视化 H22活细胞、斑马鱼和[公式:见正文]中的浓度。线虫 PD 模型。
更新日期:2020-04-30
down
wechat
bug