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MicroRNA expression profile of urinary exosomes in Type IV lupus nephritis complicated by cellular crescent
Journal of Biological Research-Thessaloniki ( IF 3.3 ) Pub Date : 2018-10-04 , DOI: 10.1186/s40709-018-0088-0
Yi Li , Xiaosong Xu , Xiaopeng Tang , Xiuwu Bian , Bingbing Shen , Hongwen Zhao , Shiyuan Luo , Zhiwen Chen , Keqin Zhang

Type IV lupus nephritis (LNIV) is a severe disease characterized by diffuse proliferative lesions, and its prognosis is worse with cellular crescent (LNIV-CC) involvement. Urinary exosomes have been shown to reflect the degree of kidney injury. This study was aimed to identify non-invasive diagnostic markers for LNIV-CC. We analysed the expression profile of microRNAs (miRNAs) isolated from urinary exosomes in patients with LNIV-CC and LNIV, and healthy individuals using high-throughput sequencing. A total of 66 differentially expressed miRNAs were identified, which were significantly enriched in 15 signalling pathways. Bioinformatic analysis revealed a co-expression network of miRNAs, predicted transcription factors and target mRNAs. Expression of three miRNAs including miR-3135b, miR-654-5p, and miR-146a-5p were further analysed and validated by reverse transcription-quantitative polymerase chain reaction. ROC analysis suggested these as candidate biomarkers for LNIV-CC. LNIV-CC has a unique miRNA expression profile of urinary exosome and complex regulatory network. miR-3135b, miR-654-5p and miR-146a-5p in urinary exosomes could be used as novel non-invasive diagnostic markers for LNIV-CC.

中文翻译:

Ⅳ型狼疮性肾炎合并细胞新月的泌尿外泌体的microRNA表达谱

Ⅳ型狼疮性肾炎(LNIV)是一种以弥漫性增生性病变为特征的严重疾病,受细胞新月形(LNIV-CC)的影响,其预后更差。尿外泌体已显示出可反映肾脏损伤的程度。这项研究旨在确定LNIV-CC的非侵入性诊断标记。我们分析了使用高通量测序从LNIV-CC和LNIV患者以及健康个体中从泌尿外泌体中分离出的microRNA(miRNA)的表达情况。总共鉴定出66个差异表达的miRNA,它们在15条信号通路中显着富集。生物信息学分析揭示了miRNA,预测的转录因子和靶标mRNA的共表达网络。三种miRNA的表达,包括miR-3135b,miR-654-5p,对miR-146a-5p和miR-146a-5p进行了反转录-定量聚合酶链反应分析。ROC分析建议将其作为LNIV-CC的候选生物标记。LNIV-CC具有泌尿外泌体和复杂调控网络的独特miRNA表达谱。泌尿外泌体中的miR-3135b,miR-654-5p和miR-146a-5p可用作LNIV-CC的新型非侵入性诊断标记。
更新日期:2018-10-04
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