Korarima (Aframomum corrorima) is a perennial and aromatic herb native and widely distributed in southwestern Ethiopia. It is known for its fine flavor as a spice in various Ethiopian traditional dishes. Few molecular studies have been performed on this species so far. In the present paper, the ISSR technique was employed to study the genetic diversity in populations of cultivated A. corrorima. Seven ISSR primers produced a total of 86 clearly scorable DNA bands. High levels of genetic diversity were detected in cultivated A. corrorima (percentage of polymorphic bands = 97.67%, gene diversity = 0.35, Shannon’s information index = 0.52). Analysis of molecular variance (AMOVA) showed that 27.47% of the variation is attributed to the variation among populations and 72.53% to the variation within populations. The Fst (0.28) value showed a significant (p < 0.0001) genetic differentiation among populations. This was supported by the high coefficient of gene differentiation (Gst = 0.32) and low estimated gene flow (Nm = 1.08). A neighbor-joining dendrogram showed that the thirteen cultivated populations were separated into three clusters, which was in good accordance with the results provided by the two dimensional and three dimensional coordinate analyses. However, the clusters did not reveal clear pattern of populations clustering according to their geographic origin. This could be due to human mediated transfer of genetic material among different localities. The genetic diversity in populations of A. corrorima from the southwestern part of Ethiopia was relatively high. This finding should be taken into account when conservation actions, management policies for the species and site identification for in situ and ex situ conservation strategies are developed. Mizan Teferi II population displayed the highest genetic diversity; this population should be considered as the key site in designing conservation strategies for this crop. In addition, Jimma I and Jimma II populations with lowest genetic diversity, should also be considered due to the putative risk of extinction that they face because of the low genetic diversity.

中文翻译：

---

埃塞俄比亚西南部耕种的非洲香菜（Aframomum corrorima（Braun）PCM Jansen]群体的遗传多样性分析，采用内部序列重复序列（ISSR）标记

Korarima（Aframomum corrorima）是多年生芳香植物，原生于埃塞俄比亚西南部。它以各种埃塞俄比亚传统菜肴中的香料而闻名。迄今为止，很少对该物种进行分子研究。本文采用ISSR技术研究了栽培拟南芥种群的遗传多样性。七个ISSR引物产生了总共86条清晰可分的DNA条带。在栽培的拟南芥中检测到高水平的遗传多样性（多态性谱带的百分比= 97.67％，基因多样性= 0.35，香农信息指数= 0.52）。分子方差分析（AMOVA）表明，27.47％的变异归因于种群之间的变异，72.53％归因于种群内的变异。Fst（0。28）值表明人群之间存在显着的（p <0.0001）遗传分化。高基因分化系数（Gst = 0.32）和低估计基因流（Nm = 1.08）支持了这一点。相邻的树状图显示，这13个耕种种群被分为三个簇，这与二维和三维坐标分析提供的结果非常吻合。但是，这些聚类并未根据其地理起源揭示出清晰的种群聚类模式。这可能是由于人类在不同地区之间介导的遗传物质转移。埃塞俄比亚西南部Corrorima种群的遗传多样性相对较高。在采取保护措施时，应考虑到这一发现，制定了物种管理和就地保护和迁地保护战略。Mizan Teferi II种群显示出最高的遗传多样性；该种群应被视为设计该作物保护策略的关键场所。此外，还应考虑遗传多样性最低的吉马一号和吉马二号种群，因为它们由于遗传多样性低而面临灭绝的风险。