The genus Nitrospira is considered to be the most widespread and abundant group of nitrite-oxidizing bacteria in many natural and man-made ecosystems. However, the ecophysiological versatility within this phylogenetic group remains highly understudied, mainly due to the lack of pure cultures and genomic data. To further expand our understanding of this biotechnologically important genus, we analyzed the high quality draft genome of “Nitrospira lenta” strain BS10, a sublineage II Nitrospira that was isolated from a municipal wastewater treatment plant in Hamburg, Germany. The genome of “N. lenta” has a size of 3,756,190 bp and contains 3968 genomic objects, of which 3907 are predicted protein-coding sequences. Thorough genome annotation allowed the reconstruction of the “N. lenta” core metabolism for energy conservation and carbon fixation. Comparative analyses indicated that most metabolic features are shared with N. moscoviensis and “N. defluvii”, despite their ecological niche differentiation and phylogenetic distance. In conclusion, the genome of “N. lenta” provides important insights into the genomic diversity of the genus Nitrospira and provides a foundation for future comparative genomic studies that will generate a better understanding of the nitrification process.

中文翻译：

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从活性污泥中分离到亚硝酸盐氧化细菌“ Nitrospira lenta ”菌株BS10的基因组序列草案。

在许多自然和人造生态系统中，硝化螺菌属被认为是亚硝酸盐氧化细菌中分布最广泛和最丰富的一类。但是，由于缺乏纯净的培养物和基因组数据，该系统发育组内的生态生理学多功能性仍被高度研究。为了进一步扩大我们对该生物技术上重要属的理解，我们分析了“ Nitrospira lenta”菌株BS10的高质量基因组草图，该菌株是II亚硝化亚型，从德国汉堡的市政废水处理厂中分离出来。“ N. “ lenta”的大小为3,756,190 bp，包含3968个基因组对象，其中3907个是预测的蛋白质编码序列。彻底的基因组注释可以重建N。lenta”核心新陈代谢，用于节能和固碳。比较分析表明，大多数代谢特征与莫斯科猪笼草和“ N. defluvii”，尽管它们具有生态位分化和系统发育距离。总之，“ N。“ lenta”提供了对硝化螺菌属基因组多样性的重要见解，并为将来的比较基因组研究奠定了基础，从而可以更好地了解硝化过程。