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Functional and taxonomic classification of a greenhouse water drain metagenome
Environmental Microbiome ( IF 7.9 ) Pub Date : 2018-10-05 , DOI: 10.1186/s40793-018-0326-y
Gamaliel López-Leal , Fernanda Cornejo-Granados , Juan Manuel Hurtado-Ramírez , Alfredo Mendoza-Vargas , Adrian Ochoa-Leyva

Microbiome sequencing has become the standard procedure in the study of new ecological and human-constructed niches. To our knowledge, this is the first report of a metagenome from the water of a greenhouse drain. We found that the greenhouse is not a diverse niche, mainly dominated by and Rodobacterales. The analysis of the functions encoded in the metagenome showed enrichment of characteristic features of soil and root-associated bacteria such as ABC-transporters and hydrolase enzymes. Additionally, we found antibiotic resistances genes principally for spectinomycin, tetracycline, and aminoglycosides. This study aimed to identify the bacteria and functional gene composition of a greenhouse water drain sample and also provide a genomic resource to search novel proteins from a previously unexplored niche. All the metagenome proteins and their annotations are available to the scientific community via http://microbiomics.ibt.unam.mx/tools/metagreenhouse/ .

中文翻译:

温室排水元基因组的功能和分类学分类

微生物组测序已成为研究新型生态和人类构建的生态位的标准程序。据我们所知,这是温室排水中的一个基因组的首次报道。我们发现,温室不是一个多样化的生态位,主要由和以轮状细菌为主。对元基因组中编码的功能的分析表明,土壤和与根相关的细菌(如ABC转运蛋白和水解酶)的特征丰富了。此外,我们发现了主要针对壮观霉素,四环素和氨基糖苷类的抗生素抗性基因。这项研究旨在鉴定温室排水样品的细菌和功能基因组成,并且还提供了一种基因组资源,可以从以前未开发的生态位中搜索新型蛋白质。
更新日期:2018-10-05
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