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Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization
Polymer-Plastics Technology and Materials ( IF 2.7 ) Pub Date : 2020-06-26 , DOI: 10.1080/25740881.2020.1784223
Dursun Saraydın 1 , H. Nursevin Öztop 2 , Ceylan Hepokur 3
Affiliation  

Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68°C and 30.44°C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH−I and NSH−I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH−I showed excellent thermal, operational and pH stability.



中文翻译:

基于海泡石纳米通道/ N-异丙基丙烯酰胺/衣康酸/丙烯酰胺的纳米复合智能水凝胶,用于固定转化酶

新型纳米复合智能水凝胶(NSH)和智能水凝胶(SH)已准备好用作转化酶的支持物。使用分光光度法和热分析方法进行表征。SH和NSH的较低临界溶液温度值分别为32.68°C和30.44°C。从在三个不同温度下进行的pH响应溶胀可以发现,与SH相比,NSH的拐点值在更高的pH下。将转化酶固定在SH和NSH(SH-1和NSH-1)上。找到了最佳的pH和温度值。计算米氏常数和最大反应速率。NSH-I具有出色的热,操作和pH稳定性。

更新日期:2020-07-24
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