ABSTRACT In this study, the effects of methanol, acetone, and ethyl acetate solvents on total phenolic content and antioxidant activity in Diospyros blancoi seeds extract were examined. Maceration extraction technique was selected in extracting the seeds. Determination of total phenolic content (TPC) and antioxidant activity used Folin-Ciocalteu and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. Radical scavenging activity data were interpreted from the calculations of the half maximal inhibitory concentration (IC50) values. TPC was found to be higher (603.43 ± 0.75 mg gallic acid equivalent/g dry powder) in D. blancoi seeds in the acetone extract compared to the methanol and ethyl acetate extracts. The highest antioxidant activity for D. blancoi seeds extracts was in methanol solvent (IC50: 2.72 μg/mL), with IC50 of ascorbic acid as a positive control was 3.34 μg/mL. These results indicate that TPC contributes to antioxidant activity in D. blancoi seeds.

中文翻译：

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三种提取溶剂对柿子总酚含量和抗氧化活性的影响

摘要 本研究考察了甲醇、丙酮和乙酸乙酯溶剂对白薯种子提取物中总酚含量和抗氧化活性的影响。在提取种子时选择浸渍提取技术。总酚含量 (TPC) 和抗氧化活性的测定分别使用 Folin-Ciocalteu 和 2,2-二苯基-1-苦基肼 (DPPH) 测定法。根据半数最大抑制浓度 (IC50) 值的计算解释自由基清除活性数据。与甲醇和乙酸乙酯提取物相比，丙酮提取物中的 D. blancoi 种子的 TPC 更高（603.43 ± 0.75 毫克没食子酸当量/g 干粉）。D. blancoi 种子提取物的最高抗氧化活性在甲醇溶剂中（IC50：2.72 μg/mL），以抗坏血酸作为阳性对照的 IC50 为 3.34 μg/mL。这些结果表明 TPC 有助于 D. blancoi 种子的抗氧化活性。