Abstract Cooking of foods and the burning of biomass and fossil fuels in stoves are the main sources of cooking fumes, with carbon black and polycyclic aromatic hydrocarbons as main components. The toxicity of carbon black and polycyclic aromatic hydrocarbons has been well studied individually, but the combined toxicity is much less understood. Carbon black can adsorb benzo(a)pyrene to form a complex which displays an altered physical form which in this study has been constructed to simulate particles in the cooking fumes and explore the combined toxic effect on rat alveolar macrophages. The complex-induced cell apoptosis and blocked cell autophagy flux compared with both individually. The mechanism of toxicity may be by intracellular reactive oxygen species generation, impairing the mitochondrial membrane potential and activating apoptosis signaling pathways. Graphical Abstract

中文翻译：

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炭黑-苯并（a）芘复合物诱导大鼠肺泡巨噬细胞凋亡和自噬阻断

摘要 食物的烹饪以及在炉灶中燃烧生物质和化石燃料是烹饪油烟的主要来源，以炭黑和多环芳烃为主要成分。炭黑和多环芳烃的毒性已分别得到很好的研究，但对组合毒性的了解却少得多。炭黑可以吸附苯并 (a) 芘形成复合物，该复合物显示出改变的物理形态，在本研究中构建该复合物以模拟烹饪油烟中的颗粒并探索对大鼠肺泡巨噬细胞的综合毒性作用。与两者单独相比，复合物诱导的细胞凋亡和阻断细胞自噬通量。毒性机制可能是通过细胞内活性氧的产生，损害线粒体膜电位并激活细胞凋亡信号通路。图形概要