Abstract The methods conventionally used for the extraction of organic pollutants in different environmental matrices are not suitable for measuring low concentrations of polycyclic aromatic hydrocarbons bioaccumulated in terrestrial snails that are both indicator of soil quality and a food item of various consumers including human being. A protocol, adapted from the QuEChERS method, originally designed for the extraction of pesticides in fruits and vegetables, in combination with gas chromatography-triple quadrupole mass spectrometry was developed to assess the bioavailability of polycyclic aromatic hydrocarbon extraction at low levels in soils to the soil quality bioindicator Cantareus aspersus. Bioaccumulation was measured in snails exposed ex situ for 28 days under controlled conditions to soils lightly or highly contaminated (sum of 16 molecules in the range 3.32–140 mg kg−1 dry weight). The method was validated (low limits of detection, linear calibration curves, good precision and trueness) and found to be efficient to show that strongly contaminated soils were not necessarily those that presented the highest bioavailable organics concentrations. This methodology offers a tool to assess the environmental risk of contaminated soils and to prioritize the management of polycyclic aromatic hydrocarbons-contaminated soils.

中文翻译：

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QuEChERS 适用于测量陆螺多环芳烃以进行风险评估

摘要 传统用于提取不同环境基质中的有机污染物的方法不适用于测量陆生蜗牛生物积累的低浓度多环芳烃，它们既是土壤质量的指标，又是包括人类在内的各种消费者的食物。根据 QuEChERS 方法改编的方案，最初设计用于提取水果和蔬菜中的农药，并结合气相色谱-三重四极杆质谱法来评估土壤中低水平多环芳烃提取到土壤的生物利用度质量生物指示剂 Cantareus aspersus。在受控条件下，将蜗牛异地暴露于轻度或重度污染的土壤（16 个分子的总和，干重范围为 3.32–140 mg kg-1）28 天后，测量了蜗牛的生物蓄积性。该方法经过验证（低检测限、线性校准曲线、良好的精密度和正确度），并发现它可以有效地表明严重污染的土壤不一定是那些具有最高生物可利用有机物浓度的土壤。这种方法提供了一种工具来评估受污染土壤的环境风险，并优先考虑多环芳烃污染土壤的管理。良好的精确度和真实性），并发现它可以有效地表明严重污染的土壤不一定是那些具有最高生物可利用有机物浓度的土壤。这种方法提供了一种工具来评估受污染土壤的环境风险，并优先考虑多环芳烃污染土壤的管理。良好的精确度和真实性），并发现它可以有效地表明严重污染的土壤不一定是那些具有最高生物可利用有机物浓度的土壤。这种方法提供了一种工具来评估受污染土壤的环境风险，并优先考虑多环芳烃污染土壤的管理。