ABSTRACT

Exploitation of biomolecular and biomimetic components on solid surfaces gain increasing importance for the design of stable functional platforms. The present study performed by quartz crystal microbalance with dissipation monitoring (QCM-D) reports on the formation of planar hybrid lipid/polymer membranes anchored to a crystalline surface (S-) layer protein lattice. In this approach, hybrid lipid/polymer vesicles were chemically bound to the S-layer protein lattice. Subsequently, to form a hybrid planar layer rupture and fusion was triggered either by (1) β–diketone – europium ion complex formation or (2) successive application of calcium ions, lowering the pH from 9 to 4, and the detergent CHAPS. As determined by QCM-D, method 1 resulted for a polymer content of 5% in a planar membrane with some imbedded intact vesicles, whereas method 2 succeeded in planar hybrid membranes with a polymer content of even up to 70%. These results provide evidence for the effective formation of planar lipid/polymer membranes varying in their composition on an S-layer protein lattice.

摘要

固体表面上生物分子和仿生成分的开发对于稳定功能平台的设计越来越重要。由耗散监测（QCM-D）的石英晶体微天平进行的本研究报告了锚定在晶体表面（S-）层蛋白晶格上的平面混合脂质/聚合物膜的形成。在这种方法中，混合脂质/聚合物囊泡化学结合到S层蛋白晶格。随后，为了形成混合平面层，破裂和融合是由（1）β-二酮-ion离子络合物的形成或（2）连续施加钙离子，将pH从9降低到4，以及洗涤剂CHAPS引发的。如通过QCM-D所确定的，方法1的结果是在带有一些完整囊泡的平面膜中，聚合物含量为5％，而方法2成功地制备了聚合物含量甚至高达70％的平面混合膜。这些结果提供了在S层蛋白质晶格上有效形成平面脂质/聚合物膜的组成变化的证据。