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Osteoclast and osteoblast responsive carbonate apatite coatings for biodegradable magnesium alloys
Science and Technology of Advanced Materials ( IF 5.5 ) Pub Date : 2020-01-31 , DOI: 10.1080/14686996.2020.1761237
Sachiko Hiromoto 1 , Sayaka Itoh 1, 2 , Naomi Noda 1 , Tomohiko Yamazaki 3 , Hideki Katayama 1 , Takaya Akashi 2
Affiliation  

ABSTRACT Corrosion-control coatings which can enhance bone formation and be completely replaced by bone are attractive for biodegradable Mg alloys. Carbonate apatite (CAp) and hydroxyapatite (HAp) coatings were formed on Mg-4 wt% Y-3 wt% rare earth (WE43) alloy as a corrosion-control and bioabsorbable coating in the coating solution with various concentrations of NaHCO3. The incorporation of carbonate group in apatite structure was examined using X-ray diffraction and Fourier transform infrared spectroscopy. Rat osteoclast precursor and MC3T3-E1 osteoblast cells were cultured on the CAp- and HAp-coated WE43 to examine the osteoclastic resorption and the alkaline phosphatase (ALP) activity, respectively. Mg ions in the used medium were quantified to examine the corrosion-control ability. The NaHCO3 addition in the solution resulted in the formation of B-type CAp in which the phosphate group of apatite structure was substituted with the carbonate group. The osteoclastic resorption was observed only for the CAp coatings as the cracking of the coatings and the corrosion of substrate WE43 strongly localized under osteoclast cell bodies. The CAp and HAp coatings significantly enhanced the ALP activity of osteoblasts. The CAp-coated WE43 specimens showed 1/5 smaller amount of Mg ion release than the uncoated WE43 on the first day of culturing osteoblasts. For the subsequent 22 days, the Mg ion release was reduced to 1/2 by the CAp coatings. In the presence of osteoclasts, the CAp coatings showed slightly lower corrosion protectiveness than the HAp coating. It was demonstrated that the CAp coatings can be a bioabsorbable and corrosion-control coating for biodegradable Mg alloys.

中文翻译:

用于可生物降解镁合金的破骨细胞和成骨细胞反应性碳酸盐磷灰石涂层

摘要 可增强骨形成并完全被骨替代的腐蚀控制涂层对于可生物降解的镁合金很有吸引力。碳酸盐磷灰石 (CAp) 和羟基磷灰石 (HAp) 涂层在 Mg-4 wt% Y-3 wt% 稀土 (WE43) 合金上形成,作为具有不同浓度 NaHCO3 的涂层溶液中的腐蚀控制和生物可吸收涂层。使用 X 射线衍射和傅里叶变换红外光谱检查磷灰石结构中碳酸酯基团的结合。大鼠破骨细胞前体和 MC3T3-E1 成骨细胞在 CAp 和 HAp 包被的 WE43 上培养,以分别检查破骨细胞吸收和碱性磷酸酶 (ALP) 活性。对所用介质中的 Mg 离子进行量化以检查腐蚀控制能力。在溶液中加入 NaHCO3 导致形成 B 型 CAp,其中磷灰石结构的磷酸基团被碳酸酯基团取代。仅对 CAp 涂层观察到破骨细胞吸收,因为涂层开裂和基底 WE43 的腐蚀强烈定位在破骨细胞体下。CAp 和 HAp 涂层显着增强了成骨细胞的 ALP 活性。在培养成骨细胞的第一天,CAp 包被的 WE43 样本显示出比未包被的 WE43 少 1/5 的镁离子释放量。在随后的 22 天里,镁离子的释放被 CAp 涂层减少到 1/2。在破骨细胞存在的情况下,CAp 涂层的腐蚀保护性略低于 HAp 涂层。
更新日期:2020-01-31
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