ABSTRACT Green synthesis of selenium nanoparticles (Se NPs) was performed by mixing Hibiscus sabdariffa (roselle plant) leaf extract with the solution of selenious acid (H2SeO3) under continuous stirring conditions resulting the roselle plant secondary metabolites conjugated Se NPs. The existence of functional groups of roselle plant secondary metabolites on the surface of prepared Se NPs was confirmed by Fourier transform infrared spectroscopy (FTIR). The formation of crystalline nanoparticles with anisotropic shape was confirmed by transmission electron microscopy (TEM) images. Furthermore, we also studied anti-oxidative and protective effects of Se NPs in streptozotocin (STZ) induced diabetes rats. These STZ induced diabetic rats were daily exposed to Se NPs or/and insulin treatment and the effect of Se NPs on the factors correlated to oxidative damage in the rat testes were evaluated. The biochemical studies showed that the Se NPs are capable to enhance the serum testosterone reduction caused due to STZ induced diabetes. In addition, Se NPs can significantly reduce the oxidative stress indicators of the testicular tissue such as nitric oxide and lipid peroxidation. However, the treatment of Se NPs on the STZ induced diabetic rats increased the activities of antioxidant enzyme as well as the glutathione content in testicular tissues. Furthermore, microscopic studies revealed that the Se NPs are capable of preventing the histological damage in the testes of STZ induced diabetic rats. Altogether, these results explained the possible effects of Se NPs in attenuating oxidative damage induced by diabetes, especially in the testicular tissue.

中文翻译：

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硒纳米颗粒的生物合成及其对链脲佐菌素诱导的糖尿病大鼠的保护、抗氧化作用

摘要 硒纳米颗粒 (Se NPs) 的绿色合成是通过在连续搅拌条件下将木槿（洛神花植物）叶提取物与亚硒酸 (H2SeO3) 溶液混合，产生与硒 NPs 结合的洛神花植物次生代谢物来进行的。傅里叶变换红外光谱 (FTIR) 证实了所制备的 Se NPs 表面存在洛神花植物次生代谢产物的官能团。通过透射电子显微镜 (TEM) 图像证实了具有各向异性形状的结晶纳米粒子的形成。此外，我们还研究了硒纳米颗粒对链脲佐菌素 (STZ) 诱导的糖尿病大鼠的抗氧化和保护作用。这些 STZ 诱导的糖尿病大鼠每天暴露于硒纳米颗粒或/和胰岛素治疗，并评估硒纳米颗粒对大鼠睾丸氧化损伤相关因素的影响。生化研究表明，Se NPs 能够增强由 STZ 诱导的糖尿病引起的血清睾酮减少。此外，Se NPs可以显着降低睾丸组织的氧化应激指标，如一氧化氮和脂质过氧化。然而，Se NPs 对 STZ 诱导的糖尿病大鼠的治疗增加了抗氧化酶的活性以及睾丸组织中的谷胱甘肽含量。此外，显微镜研究表明，Se NPs 能够预防 STZ 诱导的糖尿病大鼠睾丸的组织学损伤。共，