This study investigated the effects of three temperatures (20°C, 27°C, and 31°C) on the physiological performance (survival and growth) and cuttlebone micromorphological features (chamber number, chamber height and lamellae number) of cuttlefish Sepia pharaonis. We examined gross morphological characteristics of the internal calcareous cuttlebone to determine whether lamellar matrix was impacted by temperature. Juvenile survival was significantly affected by temperature (χ² = 54.580, df = 2, P < 0.001). Cuttlebone weight, length and width were also positively correlated with temperature. Scanning electron microscopy revealed that a single chamber structure consists of septa, lamellae and pillars. At 20°C the chamber number of 89.0 ± 10.8 was significantly higher than at 27°C with 44.8 ± 3.6 or 31°C with 47.5 ± 4.3 (Kruskal–Wallis [KW], χ²= 26.391, df = 2, P = 0.0001), whilst chamber height was lower at 20°C (KW χ²= 27.842, df = 2, P = 0.0001). Moreover, lamellae number varied among the treatments (KW χ²= 22.411, df = 2, P = 0.0002). Lamellae numbers at 20°C, 27°C and 31°C were 3–6, 6–9 and 5–8, respectively. The results indicated that the intrinsic lamellar matrix structure was significantly affected by temperature and that this effect may be used in cuttlebone growth studies.

这项研究调查了三种温度（20°C，27°C和31°C）对墨鱼乌贼墨鱼的生理性能（存活和生长）和墨鱼骨微形态特征（腔室数，腔室高度和片层数）的影响。我们检查了钙质内cut骨的总体形态特征，以确定层状基质是否受温度影响。少年存活显著受到温度的影响（χ ²  = 54.580，DF = 2，P <0.001）。骨的重量，长度和宽度也与温度呈正相关。扫描电子显微镜显示，单个腔室结构由隔垫，薄片和支柱组成。在20℃下的89.0±10.8腔室数量比在27℃下显著高于44.8±3.6或31°C 47.5±4.3（秩和检验[KW]，χ ² = 26.391，DF = 2，P  = 0.0001），而腔室高度是在20℃（KW下χ ² = 27.842，DF = 2，P  = 0.0001）。此外，薄片号码各处理间变化（KW χ ² = 22.411，DF = 2，P = 0.0002）。20°C，27°C和31°C的薄片数分别为3–6、6–9和5–8。结果表明，固有的层状基质结构受到温度的显着影响，并且这种效应可用于cut骨生长研究。