Abstract

Micro-hierarchical structured shellac films were prepared using a solution casting method with dodecyltrichloro-immobilized shellac microparticles. We evaluated two opposite surfaces of the shellac films—one a micro-hierarchical structured side and the other a neutralized shellac side using alkali ethanol—for their applications in the medical field. A bending strength test using a tensile strength testing machine and measurement of surface hardness using a scratch hardness tester indicated that the optimal composition of the base films comprised 15% shellac and 10% calcium phosphate as durability promoting agents. Further, dodecyltrichloro-immobilized shellac microparticles were synthesized and spray-deposited on the films. The solubility of shellac increased as the pH of the shellac solution increased. Analyses of cell adhesion, proliferation, and anti-thrombus efficiency were performed using a WST assay, field emission scanning electron microscope, and hemocytometer. The contact angle with the micro-hierarchical structure surface was approximately 150°. The spray-deposited shellac film yielded a lower cell and platelet adhesion rate (20%) than the untreated film. These results indicate that the micro-hierarchical structure has unique properties and that this novel superhydrophobic biodegradable shellac film can be applied as a blood/tissue-compatible, biodegradable material for implantable medical devices that need an anti-adhesion barrier.

摘要

使用溶液浇铸法用十二烷基三氯固定的虫胶微粒制备微分层结构虫胶膜。我们评估了虫胶膜的两个相对表面——一个是微分层结构的一面，另一个是使用碱性乙醇中和的虫胶面——在医学领域的应用。使用拉伸强度试验机的弯曲强度测试和使用划痕硬度测试仪的表面硬度测量表明，基膜的最佳组成包括 15% 虫胶和 10% 磷酸钙作为耐久性促进剂。此外，十二烷基三氯固定的虫胶微粒被合成并喷涂在薄膜上。虫胶的溶解度随着虫胶溶液pH值的增加而增加。分析细胞粘附、增殖、使用 WST 测定、场发射扫描电子显微镜和血细胞计数器测定抗血栓效率和抗血栓效率。与微分层结构表面的接触角约为 150°。与未处理的膜相比，喷雾沉积的虫胶膜产生较低的细胞和血小板粘附率 (20%)。这些结果表明，微分层结构具有独特的特性，这种新型超疏水性可生物降解虫胶膜可用作血液/组织相容的可生物降解材料，用于需要抗粘连屏障的植入式医疗器械。与未处理的膜相比，喷雾沉积的虫胶膜产生较低的细胞和血小板粘附率 (20%)。这些结果表明，微分层结构具有独特的特性，这种新型超疏水性可生物降解虫胶膜可用作血液/组织相容的可生物降解材料，用于需要抗粘连屏障的植入式医疗器械。与未处理的膜相比，喷雾沉积的虫胶膜产生较低的细胞和血小板粘附率 (20%)。这些结果表明，微分层结构具有独特的特性，这种新型超疏水性可生物降解虫胶膜可用作血液/组织相容的可生物降解材料，用于需要抗粘连屏障的植入式医疗器械。