This study was carried out to determine the antioxidant, antiradical and antipyretic effects and the total phenolic content of poplar-type olive oil extract of propolis (OOEP). As a result of the analysis of olive oil extract of propolis with HPLC, it was determined that the main phenolic compounds were caffeic acid phenethyl ester (CAPE), dimethoxycinnamic acid, caffeic acid, ferulic acid, p-coumaric acid, and vanillin. Propolis was extracted with cold-pressed virgin olive oil at different concentrations (control, 10, 20, 30 and 40%). The total phenolic content of these extracts was determined by Folin Ciocalteu, antioxidant activity by phosphomolybdenum, and antiradical activity by DPPH methods. It was determined that as the propolis concentration increased, total phenolic content, antioxidant, and antiradical activity also increased. The olive oil extract of propolis showed antipyretic effect in Wistar albino rats in which hyperthermia was induced by yeast. As a result, it is suggested that olive oil extract of propolis has antioxidant, antiradical and antipyretic effects; and it may be a healthier alternative to alcoholic extracts.

中文翻译：

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蜂胶橄榄油提取物的抗氧化、抗自由基和解热作用

本研究旨在确定杨树型蜂胶橄榄油提取物 (OOEP) 的抗氧化、抗自由基和解热作用以及总酚含量。用高效液相色谱法对蜂胶的橄榄油提取物进行分析，确定主要酚类化合物为咖啡酸苯乙酯 (CAPE)、二甲氧基肉桂酸、咖啡酸、阿魏酸、对香豆酸和香兰素。蜂胶是用不同浓度的冷榨初榨橄榄油（对照，10%、20%、30% 和 40%）提取的。这些提取物的总酚含量由 Folin Ciocalteu 测定，抗氧化活性由磷钼测定，抗自由基活性由 DPPH 方法测定。确定随着蜂胶浓度的增加，总酚含量、抗氧化和抗自由基活性也增加。蜂胶的橄榄油提取物在酵母诱导的高热的 Wistar 白化大鼠中显示出解热作用。因此，提示蜂胶的橄榄油提取物具有抗氧化、抗自由基和解热作用；它可能是比酒精提取物更健康的替代品。