Abstract

Nanofibers were prepared with different ratios of chitosan (CS) and polyethylene oxide (PEO) via the electrospinning technique, and tested for morphological features using scanning electron microscopy (SEM). Drug content, drug loading, release of moxifloxacin (MXF) at pH 4.7 and pH 5.5, and degree of swelling were investigated. Further characterization was accomplished via X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial activity was studied in comparison with blank nanofibers. Furthermore, wound healing was studied in Sprague-Dawley rats. A formulation consisting of CS/PEO (50/50) exhibited smooth-surfaced and beadless nanofibers having a mean diameter of 138 ± 25 nm. Drug content and drug loading in the optimized formulation, consisting of MXF, CS and PEO (0.5/2.5/2.5, w/w/w), were 99–101% and 9.1%, respectively. MXF release in 48 hours was 79.83 ± 4.2% and 94.29 ± 3.9% at pH 7.4 and pH 5.5, respectively. Moreover, degree of swelling was 133 ± 13%. The drug existed in the amorphous state and had no covalent interaction with the polymers. MXF-loaded nanofibers demonstrated greater stability, antibacterial activity and wound healing efficacy than did blank nanofibers. Accordingly, MXF-loaded CS-PEO polymeric composite nanofibers might be a potential wound dressing for effective wound healing.

摘要

通过静电纺丝技术用不同比例的壳聚糖 (CS) 和聚环氧乙烷 (PEO) 制备纳米纤维，并使用扫描电子显微镜 (SEM) 测试其形态特征。研究了药物含量、载药量、莫西沙星 (MXF) 在 pH 4.7 和 pH 5.5 下的释放以及溶胀程度。通过 X 射线衍射 (XRD)、热重分析 (TGA) 和傅里叶变换红外光谱 (FTIR) 完成进一步表征。与空白纳米纤维相比，研究了抗菌活性。此外，在 Sprague-Dawley 大鼠中研究了伤口愈合。由 CS/PEO (50/50) 组成的配方表现出表面光滑且无珠的纳米纤维，平均直径为 138 ± 25 nm。优化配方中的药物含量和载药量，由 MXF、CS 和 PEO (0.5/2.5/2.5, w/w/w)，分别为 99-101% 和 9.1%。在 pH 7.4 和 pH 5.5 下，48 小时内的 MXF 释放分别为 79.83 ± 4.2% 和 94.29 ± 3.9%。此外，溶胀度为133±13%。药物以无定形状态存在，与聚合物没有共价相互作用。与空白纳米纤维相比，装载 MXF 的纳米纤维表现出更高的稳定性、抗菌活性和伤口愈合功效。因此，载有 MXF 的 CS-PEO 聚合物复合纳米纤维可能是有效伤口愈合的潜在伤口敷料。抗菌活性和伤口愈合效果优于空白纳米纤维。因此，载有 MXF 的 CS-PEO 聚合物复合纳米纤维可能是有效伤口愈合的潜在伤口敷料。抗菌活性和伤口愈合效果优于空白纳米纤维。因此，载有 MXF 的 CS-PEO 聚合物复合纳米纤维可能是有效伤口愈合的潜在伤口敷料。