Abstract

The engineering of biomaterials provides an opportunity for developing new polymeric composites. In this context, the objective of this work is to produce and evaluate fiber obtained from mixtures of corn straw waste and polylactic acid (PLA) in vitro. Treated corn straw fiber (TCSF) was made by cutting corn straw waste and applying alkali, acid, and silane treatments. Corn straw fiber (CSF) or TCSF was combined with PLA or modified PLA (MPLA) in an electrospinning technique, to manufacture a 100–500 nm nanofiber mat. We studied morphology, mechanical properties, water resistance, biodegradability, and cytocompatibility properties of the electrospun PLA/CSF and MPLA/TCSF nanofibers. The resulting tensile properties and morphological characterizations indicated enhanced adhesion between TCSF and MPLA in the nanofiber, as well as improved water resistance and tensile strength, compared to PLA/CSF nanofiber. Regarding biocompatibility, only viable cells were found for all of the compositions, and the biocompatibility of the materials was validated by morphological analysis of cultured cells. Morphology proliferation assays showed that human keratinocyte cells grow well on the surfaces of PLA, MPLA, and their composite nanofibers. The weight loss of PLA/CSF and MPLA/TCSF composites buried in soil indicated that both were biodegradable, especially at high levels of CSF or TCSF substitution.

摘要

生物材料工程为开发新的聚合物复合材料提供了机会。在这种情况下，这项工作的目的是在体外生产和评估从玉米秸秆废料和聚乳酸 (PLA) 混合物中获得的纤维. 处理过的玉米秸秆纤维 (TCSF) 是通过切割玉米秸秆废料并应用碱、酸和硅烷处理制成的。玉米秸秆纤维 (CSF) 或 TCSF 与 PLA 或改性 PLA (MPLA) 在静电纺丝技术中结合，以制造 100-500 nm 纳米纤维垫。我们研究了电纺 PLA/CSF 和 MPLA/TCSF 纳米纤维的形态、机械性能、防水性、生物降解性和细胞相容性。由此产生的拉伸性能和形态特征表明，与 PLA/CSF 纳米纤维相比，纳米纤维中 TCSF 和 MPLA 之间的粘附力增强，并且耐水性和拉伸强度有所提高。关于生物相容性，所有组合物仅发现活细胞，并且材料的生物相容性通过培养细胞的形态学分析进行验证。形态增殖分析表明，人角质形成细胞在 PLA、MPLA 及其复合纳米纤维的表面上生长良好。埋在土壤中的 PLA/CSF 和 MPLA/TCSF 复合材料的重量损失表明两者都是可生物降解的，尤其是在高水平的 CSF 或 TCSF 替代时。