Abstract

Engineering scaffolds that can not only act as a vehicle for stem cell delivery but also support axon regeneration, may significantly improve the treatment outcome of spinal cord injury (SCI). In this study, we developed chitosan scaffold containing human endometrial stem cells (hEnSCs) and platelet-rich plasma (PRP) to provide a controlled environment for facilitating cell ingrowth and differentiation and also to persuade axon nervous tissue repair. The results showed that 80 µL PRP-loaded chitosan scaffold has excellent hEnSCs viability and can promote the regeneration of nerve fibers and angiogenesis and prevent the formation of scar tissue in the repair of SCI.

摘要

工程支架不仅可以作为干细胞输送的载体，还可以支持轴突再生，可以显着改善脊髓损伤 (SCI) 的治疗结果。在这项研究中，我们开发了含有人类子宫内膜干细胞 (hEnSCs) 和富含血小板的血浆 (PRP) 的壳聚糖支架，为促进细胞向内生长和分化提供受控环境，并说服轴突神经组织修复。结果表明，80 μL负载PRP的壳聚糖支架具有优异的hEnSCs活力，在SCI修复中可促进神经纤维再生和血管生成，防止瘢痕组织形成。