Abstract

In the present study silver nanoparticles were synthesized from Elaeodendron croceum stem bark and leaf extracts (ECB-AgNPs and ECL-AgNPs respectively) and were characterized using UV-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and transmission electron microscopy. The antioxidant activity was determined by evaluating the effect on DPPH and ABTS. The activities against selected Gram negative and Gram-positive bacteria as well as MCF-7 and MDA-MB-231 cells were also carried out. The nanoparticles obtained were stable aqueous dispersive, crystalline, and spherical shaped with average particle size of 22.26 nm and 10.01 nm for ECB-AgNPs and ECL-AgNPs respectively. The X-ray Diffraction (XRD) patterns revealed four diffraction peaks at 32.24°, 48.11°, 58.64°, and 77.47° that was indexed to (111), (200), (220), and (311) planes fcc. However, the minimum inhibitory concentrations and IC₅₀ values were higher than the standards used. In summary, a single step method of synthesizing silver nanoparticles from Elaeodendron croceum stem bark and leaf is reported and may serve as potential source of antimicrobial and anticancer agents.

摘要

在本研究中，从大花蜡木合成了银纳米颗粒茎皮和叶提取物（分别为ECB-AgNPs和ECL-AgNPs），并使用紫外-可见光谱，傅里叶变换红外光谱，X射线衍射和透射电子显微镜进行表征。通过评估对DPPH和ABTS的影响来确定抗氧化活性。还对选定的革兰氏阴性和革兰氏阳性细菌以及MCF-7和MDA-MB-231细胞进行了活性。获得的纳米颗粒是稳定的水分散体，晶体和球形，其ECB-AgNPs和ECL-AgNPs的平均粒径分别为22.26 nm和10.01 nm。X射线衍射（XRD）图谱显示在32.24°，48.11°，58.64°和77.47°处有四个衍射峰，分别指向（111），（200），（220）和（311）平面fcc。但是，最低抑菌浓度和IC_50个值高于使用的标准。总而言之，据报道一步法从大花古兰茎皮和叶中合成银纳米颗粒，可以作为抗菌剂和抗癌剂的潜在来源。