The use of microwells sealed with lipid bilayers is promising for biodevices that utilize protein functions. We have fabricated microwell structures on a Si substrate sealed with lipid bilayers. We have investigated Ca²⁺ ion transport through α‐hemolysin channels with fluorescent microscopy. However, problems remain as regards achieving the detection of smaller signal such as receptor channels. Ion leakage through the interfacial water layer between the lipid bilayer and the substrate is one of the major hurdles to be overcome. In this paper, we present the mechanism of the ion leakage behavior and then an improved microwell structure that uses bovine serum albumin coating on the device surface to prevent ion leakage from/into the microwells.

中文翻译：

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使用用人造脂质双层密封的微孔的生物设备：通过蛋白质涂层改善密封性能

对于利用蛋白质功能的生物装置来说，用脂质双层密封的微孔的应用是有希望的。我们在用脂质双层密封的Si基板上制造了微孔结构。我们用荧光显微镜研究了通过α-溶血素通道的Ca ²⁺离子转运。然而，关于实现对诸如接收器通道之类的较小信号的检测仍然存在问题。通过脂质双层和底物之间的界面水层的离子泄漏是要克服的主要障碍之一。在本文中，我们介绍了离子泄漏行为的机理，然后介绍了一种改进的微孔结构，该结构在设备表面上使用牛血清白蛋白涂层来防止离子从微孔中渗入/渗入微孔中。