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Inoculation of arbuscular mycorrhizal fungi and phosphorus addition increase coarse mint (Plectranthus amboinicus Lour.) plant growth and essential oil content
Rhizosphere ( IF 3.7 ) Pub Date : 2020-05-30 , DOI: 10.1016/j.rhisph.2020.100217
Everton Merlin , Elisangela Melato , Emerson Luiz Botelho Lourenço , Ezilda Jacomassi , Arquimedes Gasparotto Junior , Rayane Monique Sete da Cruz , Joice Karina Otênio , Camila da Silva , Odair Alberton

The coarse mint (Plectranthus amboinicus Lour.) is a medicinal plant that produces essential oil; however, it is still little studied the effects of effects of arbuscular mycorrhizal fungi species on growth and essential oil content and its composition in P. amboinicus. Therefore, this study aimed to evaluate Rhizophagus clarus and Claroideoglomus etunicatum under low and high phosphorus conditions and its effects in plant growth and the essential oil content and chemical composition in coarse mint. The experimental design was completely randomized with 8 replicates per treatment in a 3 × 2 factorial (without and with two arbuscular mycorrhizal fungi, two levels of phosphorus (at 20 or 200 mg dm−3 of substrate). We evaluated spore density in the substrate and root colonization by arbuscular mycorrhizal fungi, an also shoot and root phosphorus content and nitrogen in the shoots, plant growth, essential oil content and its chemical composition. Spore density and root colonization by arbuscular mycorrhizal fungi were higher in the inoculated treatments. The shoot dry matter, root dry matter and total dry matter increased with arbuscular mycorrhizal fungi inoculation, mainly with the inoculation of R. clarus and high addition of phosphorus to the substrate. Inoculation of arbuscular mycorrhizal fungi and/or the addition of phosphorus in the substrate increased phosphorus and nitrogen content in the plant. The essential oil content was higher with R. clarus and with low phosphorus addition (0.61%) and 18 compounds were identified in essential oil, being carvacrol (38.48–51.07%), followed by trans-Caryophyllene, α-Bergamotene and α-Humulene the major constituents. It was concluded that plant growth increased with R. clarus inoculation and high phosphorus condition and R. clarus inoculation increased essential oil content.



中文翻译:

接种丛枝菌根真菌和添加磷可增加粗薄荷(Plectranthus amboinicus Lour。)植物的生长和精油含量

粗薄荷(Plectranthus amboinicus Lour。)是一种药用植物,可生产精油;然而,关于丛枝菌根真菌种类对竹节菌生长和精油含量及其组成的影响的研究还很少。因此,本研究旨在评估低磷和高磷条件下的根瘤菌克拉克球菌及其对植物生长以及粗薄荷中精油含量和化学成分的影响。实验设计完全随机化,在3×2阶乘中每次处理重复8次(不使用和同时使用两种丛枝菌根真菌,两种水平的磷(在20或200 mg dm -3时)基材)。我们评估了丛枝菌根真菌在基质中的孢子密度和根定植,还发现了芽和根中的磷含量和芽中的氮,植物生长,精油含量及其化学成分。在接种的处理中,丛枝菌根真菌的孢子密度和根定植更高。丛枝菌根真菌接种增加了枝干,根系干物质和总干物质的增加,主要是接种了克拉克红杆菌和向基质中大量添加磷。接种丛枝菌根真菌和/或在基质中添加磷可增加植物中的磷和氮含量。R. clarus的精油含量更高低磷添加量(0.61%)和精油中鉴定出18种化合物,其中香芹酚(38.48-51.07%),其次是反式-石竹烯,α-香柠檬烯和α-腐殖烯。得出的结论是,随着克拉克菌的接种和高磷条件下植物的生长增加,而克拉克菌的接种增加了精油含量。

更新日期:2020-05-30
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