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Motility and biofilm production involved in the interaction of phosphate solubilizing endophytic strains with peanut, maize and soybean plants
Rhizosphere ( IF 3.7 ) Pub Date : 2020-07-06 , DOI: 10.1016/j.rhisph.2020.100228
Cinthia Tamara Lucero , Graciela Susana Lorda , Liliana Mercedes Ludueña , María Soledad Anzuay , Tania Taurian

Endophytic plant growth promoting bacteria are of great interest since the ability to colonize the internal tissues of plants gives it an adaptive advantage. To confer any beneficial effect on the plant, bacteria must present a successful colonization and thus be competent in the rhizosphere and finally infect internal tissues. In this sense, bacterial motility is an essential property involved in plant-microorganism interaction where bacteria can colonize the root and at the same time form biofilms. Within plant beneficial bacteria those that present phosphate solubilizing activity are of great interest due to the contribution of phosphorus to plants. The objective of this work was to evaluate the motility and biofilm formation properties of the strains Serratia sp. S119 and Enterobacter sp. J49 growing in the presence of peanut, maize and soybean root exudates. Results obtained indicated that both strains have flagella, possess swimming, swarming and twitching motilities and showed differential chemotactic attraction against root exudates. In addition, the strains under study showed the ability to form biofilms, being this ability greater in minimal media, in which a greater decrease was also seen by the addition of root exudates in the first hours of growth. The changes produced by the addition of root exudates in the chemotaxis and biofilm formation of the strains suggests that compounds released by the plants are detected by these bacteria and could be part of the molecular dialogue involved in their interaction with the roots of plant.



中文翻译:

溶解性磷酸盐内生菌株与花生,玉米和大豆植物相互作用的动力和生物膜产生

内生植物生长促进细菌引起极大兴趣,因为其能够在植物内部组织中定殖的能力使其具有适应性优势。为了赋予植物任何有益的作用,细菌必须成功地定植,并因此能够在根际中起作用,并最终感染内部组织。从这个意义上说,细菌的运动性是植物与微生物相互作用所必需的一种特性,其中细菌可以在根部定植,并同时形成生物膜。在植物有益细菌中,由于磷对植物的贡献,具有磷酸盐增溶活性的细菌引起人们极大的兴趣。这项工作的目的是评估菌株沙雷氏菌的运动性和生物膜形成特性。S119和肠杆菌sp。J49在花生,玉米和大豆根系分泌物存在下生长。获得的结果表明,两种菌株均具有鞭毛,具有游动,成群和抽动的功能,并且对根系分泌物表现出不同的趋化吸引力。此外,正在研究的菌株显示出形成生物膜的能力,在最小培养基中该能力更大,其中在生长的最初几个小时内添加根系分泌物也观察到更大的降低。通过在菌株的趋化性和生物膜形成中添加根系分泌物而产生的变化表明,植物释放的化合物被这些细菌检测到,并且可能是与植物根部相互作用所涉及的分子对话的一部分。

更新日期:2020-07-06
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