Abstract In this study, an intra-laboratory assessment was carried out to establish the effectiveness of a method for the detection of TiO2 engineered nanoparticles (ENPs) present in sunscreen containing nano-scale TiO2 and a higher nanometer-range (approx. 200–500 nm) TiO2, as well as iron oxide particles. Three replicate measurements were performed on five separate days to generate the measurement uncertainties associated with the quantitative asymmetrical flow field-flow fractionation (AF4) measurement of the hydrodynamic radius rh,mode1 (MALS), rh,mode1 (ICP-MS), rh,mode2 (ICP-MS), and calculated mass-based particle size distribution (d10, d50, d90). The validation study demonstrates that the analysis of TiO2 ENPs present in sunscreen by AF4 separation-multi detection produces quantitative data (mass-based particle size distribution) after applying the sample preparation method developed within the NanoDefine project with uncertainties based on the precision (uIP) of 3.9–8.8%. This method can, therefore, be considered as the method with a good precision. Finally, the bias data shows that the trueness of the method (ut = 5.5–52%) can only be taken as a proxy due to the lack of a sunscreen standard containing certified TiO2 ENPs.

中文翻译：

---

基于多检测器非对称流场-流动分级检测防晒霜中二氧化钛纳米粒子的方法的实验室内评估

摘要 在本研究中，进行了一项实验室内评估，以确定检测防晒霜中含有纳米级 TiO2 和更高纳米范围（约 200-500 nm) TiO2，以及氧化铁颗粒。在五个不同的日子里进行了三次重复测量，以产生与流体动力学半径 rh,mode1 (MALS)、rh,mode1 (ICP-MS)、rh、rh、模式 2 (ICP-MS) 和计算的基于质量的粒度分布（d10、d50、d90）。验证研究表明，在应用 NanoDefine 项目中开发的样品制备方法后，通过 AF4 分离-多重检测对防晒霜中存在的 TiO2 ENP 进行分析会产生定量数据（基于质量的粒度分布），但不确定度基于精度 (uIP) 3.9–8.8%。因此，该方法可以被认为是具有良好精密度的方法。最后，偏差数据表明该方法的正确性（ut = 5.5-52%）只能作为替代，因为缺乏包含经认证的 TiO2 ENP 的防晒标准。