This research aims to study the therapeutic effect of dexmedetomidine on type 2 diabetic rats reperfusion injury model. A rat model of type 2 diabetes was induced by high-fat and high-sugar feed, and a reperfusion rat model was obtained by ligating the left anterior descending branch and reperfusion. The diabetic rat model sham operation group was used as the control group (DM-S), the rats were divided into diabetic reperfusion group (DM-IR) and dexmedetomidine treatment group (DM-D), dexmedetomidine combined with DM-DW (DM-DW), and Wortmannin treatment group (DM-W). In this study, the MB isoenzyme of creatine kinase (CK-MB) concentration was determined by ELISA competition method, superoxide dismutase (SOD) activity in rat plasma was detected by superoxide dismutase WST-1, and the content of malonaldehyde (MDA) in rat plasma was determined by thiobarbituric acid method. Besides, Western Blot was used to detect the expression changes of Akt, P-Akt, GSK-3β, and P-GSK-3β in rat myocardial tissue. The results showed that the content of CK-MB and SOD in DM-D group was significantly higher than that in DM-IR group (P < 0.05), and the content of MAD was significantly lower than that in DM-IR group (P < 0.05). The content of CK-MB and SOD in DM-DW group and DM-W group were significantly higher than that in DM-D group (P < 0.05), and the content of MDA was significantly lower than that in DM-D group (P < 0.05). However, expression of p-Akt and p-GSK-3β protein in DM-D group was significantly higher than that in DM-DW group and DM-W group (p < 0.05). Accordingly, dexmedetomidine can play a role in treating myocardial ischemia-reperfusion injury in type 2 diabetes by regulating the expression of related genes in the P13K/Akt signaling pathway.

本研究旨在研究右美托咪定对2型糖尿病大鼠再灌注损伤模型的治疗作用。高脂高糖饲料诱导成2型糖尿病大鼠模型，结扎左前降支再灌注获得再灌注大鼠模型。糖尿病模型假手术组为对照组（DM-S），分为糖尿病再灌注组（DM-IR）和右美托咪定治疗组（DM-D），右美托咪定联合DM-DW（DM）。 -DW）和Wortmannin治疗组（DM-W）。在这项研究中，通过ELISA竞争法测定了肌酸激酶（CK-MB）的MB同工酶浓度，通过超氧化物歧化酶WST-1检测了大鼠血浆中的超氧化物歧化酶（SOD）活性，用硫代巴比妥酸法测定大鼠血浆丙二醛（MDA）含量。此外，Western Blot用于检测大鼠心肌组织中Akt，P-Akt，GSK-3β和P-GSK-3β的表达变化。结果表明，DM-D组的CK-MB和SOD含量明显高于DM-IR组（P  <0.05），且MAD含量明显低于DM-IR组（P  <0.05）。DM-DW组和DM-W组的CK-MB和SOD含量均显着高于DM-D组（P  <0.05），MDA的含量显着低于DM-D组（P <0.05）。P  <0.05）。然而，DM-D组中p-Akt和p-GSK-3β蛋白的表达显着高于DM-DW组和DM-W组（p  <0.05）。因此，右美托咪定可通过调节P13K / Akt信号通路中相关基因的表达，在2型糖尿病的心肌缺血-再灌注损伤中发挥作用。