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Glycation of heme-protein, “myoglobin” by 3-deoxyglucosone: Implications in immunogenicity
Journal of King Saud University-Science ( IF 3.8 ) Pub Date : 2020-05-07 , DOI: 10.1016/j.jksus.2020.04.019
Rongfang Tang , Mohammad Faisal , Abdulrahman A. Alatar , Asma N. Alsaleh , Mohd Saeed , Saheem Ahmad

The Reactive carbonyl species (RCS) like, 3-deoxyglucosone (3-DG) is a highly reactive intermediate of the glycation reaction which may result into the formation of protein advanced glycation end-products (AGEs), like myoglobin-AGEs (Mb-AGEs) which further lead to the structural perturbations. In the present work, we studied the glycation of myoglobin (Mb) by physico-chemical techniques and the resulting immunogenicity by ELISA techniques. The level of free lysine and arginine determined was found to be significantly decreased indicating the establishment of the glycation reaction. The glycated myoglobin (G-Mb) shows the increased absorbance at 280 nm and enhanced fluorescence emission at 350 nm excitation wavelengths. The levels of ketoamine and hydroxymethylfurfural (HMF) were recorded to be enhanced in G-Mb when compared to its native conformer. Furthermore, the New-Zealand white female rabbits immunized with native and its glycated analogue ‘G-Mb’ induced high titer antibodies as compared to the native one.



中文翻译:

3-脱氧葡糖酮糖化血红蛋白“肌红蛋白”:对免疫原性的影响

反应性羰基物质(RCS)像3-脱氧葡萄糖苷(3-DG)是糖基化反应的高反应性中间体,可能导致形成蛋白质高级糖基化终产物(AGEs),例如肌红蛋白-AGEs(Mb- AGEs)进一步导致结构扰动。在本工作中,我们通过理化技术研究了肌红蛋白(Mb)的糖基化作用,并通过ELISA技术研究了所得的免疫原性。发现确定的游离赖氨酸和精氨酸水平显着降低,表明糖基化反应的建立。糖化肌红蛋白(G-Mb)在280 nm处显示出增加的吸光度,并在350 nm激发波长下显示出增强的荧光发射。据记录,与天然构象相比,G-Mb中酮胺和羟甲基糠醛(HMF)的含量有所提高。

更新日期:2020-05-07
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