Mastitis an inflammation of mammary gland in dairy herds is a key concern of economic losses worldwide caused by bacteria and its toxins. In this study, we investigated pro-inflammatory and anti-inflammatory cytokines (IL-2, IL-1β, IL-6, TGF-β, IL-10, TNF-α), lactoferrin and albumin, and milk composition in normal dairy cows and lactating cows with symptoms of sub-clinical and clinical mastitis. Lactating cows with clinical mastitis showed marked increase in IL-2, IL-6, IL-1β, TNF-α and a decrease in anti-inflammatory levels of TGF-β, IL-10, milk parameters fat, protein, SNF, lactose and increase in pH compared to normal lactating cows. Subclinical lactating cows showed significant alteration in TNF-α, IL-1β when compared to normal lactating cows. There was no significant difference between IL and 2 and IL-6 in normal and subclinical lactating cows. The subclinical cows also did not exhibit significant difference in TGF-β, albumin, milk fat, protein and pH when compared with normal lactating cows. Findings in the present study indicate that cytokines together with proteins lactoferrin and albumin can be considered as prospective markers in early detection of subclinical and clinical mastitis.

乳腺炎是奶牛群中的乳腺发炎，是细菌及其毒素在全球范围内造成的经济损失的关键问题。在这项研究中，我们调查了正常乳制品中促炎和抗炎细胞因子（IL-2，IL-1β，IL-6，TGF-β，IL-10，TNF-α），乳铁蛋白和白蛋白以及牛奶成分患有亚临床和临床乳腺炎症状的奶牛和哺乳奶牛。患有临床乳腺炎的泌乳牛表现出IL-2，IL-6，IL-1β，TNF-α的显着增加以及TGF-β，IL-10，乳汁脂肪，蛋白质，SNF，乳糖的抗炎水平降低与正常泌乳牛相比，pH值增加。与正常泌乳母牛相比，亚临床泌乳母牛表现出TNF-α，IL-1β的显着改变。正常和亚临床泌乳母牛的IL与2和IL-6之间无显着差异。与正常泌乳的母牛相比，亚临床母牛的TGF-β，白蛋白，乳脂，蛋白质和pH值也没有显着差异。在本研究中的发现表明，细胞因子与蛋白质乳铁蛋白和白蛋白一起可以被认为是早期检测亚临床和临床乳腺炎的前瞻性标志。