Background

Based on “gut-brain axis” theory, we employed a rat 6-OHDA lesion model to examine the impact of gut-acupuncture on motor symptoms, the abundance of DA neurons in the SN, the hemodynamic characteristics of the carotid artery and SNH.

Methods

6-OHDA-induced PD rats were treated with gut-acupuncture daily, starting day 15 after surgery. Mobility of the limbs was evaluated, abundance of TH + DA neurons in the SN detected, The diameter of carotid arteries and hemodynamic parameters measured by cervical vascular ultrasound CVUS, and TCS utilized to examine SNH.

Results

Our data show that gut-acupuncture correlates with significantly increased abundance of TH, a marker of DA neurons compared to untreated rats. Furthermore the area of SNH in the injured side of the acupuncture group was significantly reduced. PSV of LCCA, LICA, RICA showed that were significantly improved i.e. decreased in the acupuncture group, while the diameter of LICA and RCCA in acupuncture group was narrower. We also found that the PSV was significantly increased and the vascular diameter narrowed in LCCA and LICA during treatment, whereas after removing the acupuncture needle the PSV decreased and the vascular diameter widened.

Conclusions

Gut-acupuncture can reduce SNH and influence TH abundance in the SN which correlated with changes of hemodynamic characteristics of the lesioned side. We suggest a regulatory mechanism which may affect the vagus nerve through the ENS and cause the change of cervical hemodynamics. It further induces low oxygen tension microenvironment conducive to the proliferation of neural stem cells, which leads to the enrichment of TH in PD model rats of acupuncture group.

中文翻译：

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颈动脉血流动力学特征变化对6-OHDA致肠刺帕金森病模型大鼠的影响

背景

基于“肠脑轴”理论，我们采用了大鼠6-OHDA损伤模型，研究了肠道针刺对运动症状，SN中DA神经元丰富，颈动脉和SNH的血液动力学特征的影响。

方法

从术后第15天开始，每天用肠针治疗6-OHDA诱导的PD大鼠。评估四肢的活动性，检测SN中的TH + DA神经元的数量，通过颈血管超声CVUS测量颈动脉直径和血液动力学参数，并使用TCS检查SNH。

结果

我们的数据表明，与未经治疗的大鼠相比，肠胃针灸与DA神经元标志物TH的丰度显着增加有关。此外，针灸组受伤侧的SNH面积明显减少。LCCA，LICA，RICA的PSV显示，针刺组显着改善，即降低，而针刺组的LICA和RCCA直径更窄。我们还发现，在治疗期间，LCCA和LICA中PSV显着增加，血管直径变窄，而在拔出针灸针后，PSV减小并且血管直径变宽。

结论

针刺可以减少SN中的SNH并影响TH中的TH含量，这与病变侧的血流动力学特征相关。我们建议一种调节机制，可能通过ENS影响迷走神经并引起子宫颈血流动力学改变。进一步诱导低氧微环境有利于神经干细胞的增殖，从而导致针刺组PD模型大鼠体内TH的富集。