Purpose

The aim of this study was to analyze the effects of mechanical vibration on the histomorphometry and oxidative stress of oophorectomized rats.

Method

Seventy-two Wistar rats were randomized to Pseudoophorectomy (P) and Oophorectomy (O) and subdivided into untreated animals and euthanized after four (P4 and O4) and eight (P8 and O8) weeks and animals treated during four (PT4 and OT4) and eight (PT8 and OT8) weeks. The treatment consisted of use of whole-body vibration for 10 min, three times a week. After euthanasia, the soleus muscle was collected. The general morphological analysis was performed in the right soleus muscle and then the cross-sectional area, the largest and the smallest diameter of the muscle fiber in 100 fibers per muscle, also the nuclei and capillary/fiber ratios, and percentage of connective tissue were measured. The left soleous was used for oxidative stress analysis.

Results

PT4 presented higher values in cross-sectional area than P4 and PT8, while O8 was lower than O4, P8 and OT8; for the fiber diameters, the oophorectomized animals had lower values than the pseudo-oophorectomized animals and the treatments values higher than the ones that had no treatment. In oxidative stress, O8 and OT8 presented higher lipoperoxidation, without any alterations to the activities of superoxide dismutase, catalase and cholinesterase.

Conclusion

Whole-body vibration induced muscle hypertrophy in the pseudo-oophorectomized rats after four weeks, as well as being able to reverse the changes caused by the surgery in eight weeks in that variable.

中文翻译：

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全身振动对输卵管切除大鼠比目鱼肌的短期影响：动物模型的组织形态计量学分析和氧化应激。

目的

这项研究的目的是分析机械振动对卵巢切除的大鼠组织形态学和氧化应激的影响。

方法

将72只Wistar大鼠随机分为假性眼睑切除术（P）和耳蜗切除术（O），再细分为未经治疗的动物，并在四周（P4和O4）和八周（P8和O8）进行安乐死，并在四周（PT4和OT4）和八周（PT8和OT8）。治疗包括每周三次，全身振动10分钟。安乐死后，收集比目鱼肌。在右比目鱼肌中进行一般的形态分析，然后以每根肌肉中100条纤维的横截面积，最大和最小直径的肌纤维，核和毛细血管/纤维的比率以及结缔组织的百分比进行分析。测量。左肌用于氧化应激分析。

结果

PT4的截面积值高于P4和PT8，而O8的值低于O4，P8和OT8。对于纤维直径，经去卵巢切除的动物的值低于假经去卵巢的动物，且处理值高于未进行处理的动物。在氧化应激中，O8和OT8表现出较高的脂过氧化作用，而对超氧化物歧化酶，过氧化氢酶和胆碱酯酶的活性没有任何改变。

结论

四周后，全身振动导致假性去卵巢切除大鼠的肌肉肥大，并且能够在八周内逆转由手术引起的变化。