A method using ultrahigh-performance liquid chromatography in combination with high-resolution time-of-flight mass spectrometry was developed for simultaneous identification and determination of the active ingredients (cetylpyridinium chloride, benzydamine, benzocaine, lidocaine, and tetracaine) in Septolete, Calgel, and Novosept Forte preparations. Analytes were identified using retention times, exact masses of ions, and agreement of isotopic distribution patterns (mSigma). The detection limits and limits of quantitation were 0.001 – 0.005 and 0.005 – 0.01 ng/mL, respectively, for all studied analytes. The ranges of determined contents were 0.01 – 50 and 0.005 – 10 ng/mL. The method was verified using actual preparations. The relative standard deviations of the analytical results were <0.05.

中文翻译：

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Septolete、Calgel 和 Novosept Forte 制剂中活性成分的同时 LC-MS 鉴定和测定

开发了一种将超高效液相色谱与高分辨率飞行时间质谱联用的方法，用于同时鉴定和测定 Septolete、Calgel 中的活性成分（氯化十六烷基吡啶、苄达明、苯佐卡因、利多卡因和丁卡因），和 Novosept Forte 制剂。使用保留时间、离子的精确质量和同位素分布模式的一致性 (mSigma) 来鉴定分析物。对于所有研究的分析物，检测限和定量限分别为 0.001 – 0.005 和 0.005 – 0.01 ng/mL。测定的含量范围为 0.01 – 50 和 0.005 – 10 ng/mL。使用实际制剂验证了该方法。分析结果的相对标准偏差<0.05。