A sensitive and reliable high-performance thin-layer chromatography method has been developed for the simultaneous estimation of bavachin, bakuchiol, and psoralen in Psoralea corylifolia seeds. The methanolic extract of the seeds was applied on a silica gel G 60 F₂₅₄ plate. The plate was developed using toluene–ether, 1:1, saturated with 10% acetic acid (v/v) as the mobile phase and detection and quantification were performed by densitometric scanning at 254 nm. The correlation coefficients were found to be 0.999, 0.999, and 0.999 for bavachin, bakuchiol, and psoralen, respectively, in the developed method. The accuracy of the method was confirmed by conducting recovery studies at different levels using the standard addition method. The average recovery of bavachin, bakuchiol, and psoralen was found to be close to 98% confirming the accurateness of the method. The proposed method offers a new, sensitive, specific, and precise gauge for quantification of bavachin, bakuchiol, and psoralen representing all the categories of bioactive constituents in P. corylifolia.

中文翻译：

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一种经过验证的高效薄层色谱方法，用于定量测定补骨脂种子中的巴瓦钦，补骨脂酚和补骨脂素

甲灵敏和可靠的高性能薄层色谱法已经发展为补骨脂异黄酮，补骨脂酚的同时估计，并在补骨脂补骨脂种子。将种子的甲醇提取物施加在硅胶G 60 F ₂₅₄板上。该板使用甲苯-乙醚（1：1，用10％乙酸（v / v）饱和）显影）作为流动相，并通过254 nm的光密度扫描进行检测和定量。在开发的方法中，巴瓦钦，补骨脂酚和补骨脂素的相关系数分别为0.999、0.999和0.999。通过使用标准添加方法在不同水平进行回收率研究，确认了该方法的准确性。发现巴瓦钦，爆裂酚和补骨脂素的平均回收率接近98％，证实了该方法的准确性。所提出的方法提供一个新的，灵敏，特异和补骨脂异黄酮，补骨脂酚的量化精确计，和补骨脂素代表生物活性成分的所有类别P.补骨脂。