The metabolite contents of the plants may vary with season and geographic variations, and this induces the biological potential of the plant species. The present study aimed to identify the elite chemotype(s) of Plumbago zeylanica, collected from the natural habitat of Gangetic plains on the basis of plumbagin content. The effect of intra-specific variability in the plumbagin content among the population was estimated by using high-performance thin-layer chromatography (HPTLC) densitometric method. Roots of thirteen naturally occurring populations of P. zeylanica from the entire range were collected, covering the wide topography from foothills up to the highest peak. The method developed for quantification of plumbagin was validated and found to be linear, specific and accurate with precision and accuracy. LOD and LOQ were 1.04 and 3.16 ng/spot. Precision studies (both inter-day and intra-day) were within the standard limit of API, with RSD (%) less than 3%. Quantification of plumbagin within population reveals that it varies from 0.0048 ± 0.0005 μg (NBP-22) to 0.1696 ± 0.003 μg (NBC-25) percent on per dry weight basis. Analysis of variance (ANOVA) suggests that there is a significant difference in plumbagin content among the population. UPGMA cluster analyses based on plumbagin content bifurcate the populations into two major clusters, representing the variation in metabolite content. The first major cluster having NBP-25 and NBP-18 accessions, NBP-25 having maximum plumbagin content was collected from Bankura, West Bengal. The study suggests the presence of chemotaxonomic variation among the population which may be due to the change in environmental/phytogeographical factors.

中文翻译：

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玉米（Plumbago zeylanica Linn）代谢物变异性的研究。从印度恒河平原的不同地方收集的

植物的代谢物含量可能随季节和地理变化而变化，这会诱发植物物种的生物潜能。本研究的目的是确定从铅球藻含量的基础上从恒河平原的自然栖息地收集的西兰花的精英化学型。通过使用高效薄层色谱（HPTLC）密度测定法评估种群中铅蛋白含量的种内变异性影响。zeylanica的13个自然种群的根收集了整个山脉的地形，涵盖了从山麓到最高峰的宽阔地形。验证了开发用于定量铅白蛋白的方法，结果证明该方法是线性的，特异性的，准确的，并且具有精密度和准确性。LOD和LOQ为1.04和3.16 ng / spot。精密度研究（日间和日间）均在API的标准范围内，RSD（％）小于3％。群体中铅青霉素的定量显示，基于每干重，铅青霉素的含量范围为0.0048±0.0005μg（NBP-22）至0.1696±0.003μg（NBC-25）％。方差分析（ANOVA）表明，人群中铅锌的含量存在显着差异。基于铅白蛋白含量的UPGMA聚类分析将种群分为两个主要的聚类，代表了代谢物含量的变化。从西孟加拉邦班库拉（Bankura）收集到第一个具有NBP-25和NBP-18的主要簇，NBP-25具有最大的羽扇豆苷含量。该研究表明，人群中存在化学分类学差异，这可能是由于环境/植物地理因素的变化所致。