Superfine pulverization and ordinary pulverization were used to obtain powders with different particle sizes in different parts of Fructus rosae laxae. High-performance thin-layer chromatography (HPTLC) was used to carry out a simple, fast, qualitative, and quantitative analysis of vitamin C in Fructus rosae laxae. The effects of superfine pulverization and ordinary pulverization on the vitamin C content in the whole fruit, pulp, and seed of Fructus rosae laxae were compared. A G F₂₅₄ silica gel thin-layer plate was utilized, and ethyl acetate, anhydrous ethanol, and water in (10:6:3.75, v/v) ratio were used as a developer. The scanning wavelength was 273 nm. Results showed that the Rf value of vitamin C was 0.61, the linear range was 0.25–1.2 μg, and the correlation coefficient was 0.9959, indicating good linear correlation. Precision RSD was < 2%, indicating good instrument precision. The sample was stable within 40 min at room temperature, and the RSD was < 3%. The average recovery of vitamin C was 98.39%, and the established method was reliable. The content of vitamin C in the whole fruit, pulp, and seed of Fructus rosae laxae after superfine pulverization was higher than that obtained from ordinary pulverization. The content of vitamin C in the pulp was the highest after superfine pulverization.

中文翻译：

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超微粉碎技术对玫瑰果中维生素C含量测定的影响

使用超细粉碎和普通粉碎在玫瑰果的不同部位获得具有不同粒径的粉末。高性能薄层色谱法（HPTLC ）用于对玫瑰果中的维生素C进行简单，快速，定性和定量的分析。比较了超细粉碎和普通粉碎对玫瑰果整个果实，果肉和种子中维生素C含量的影响。使用AGF ₂₅₄硅胶薄层板，并且以（10：6：3.75，v / v）比率的乙酸乙酯，无水乙醇和水作为显影剂。扫描波长为273nm。结果显示，维生素C的Rf值为0.61，线性范围为0.25 –1.2μg，相关系数为0.9959，表明线性相关性良好。精密度RSD <2％，表明仪器具有良好的精密度。样品在室温下40分钟内稳定，RSD <3％。维生素C的平均回收率为98.39％，建立的方法可靠。超细粉碎后的蔷薇果的整个果实，果肉和种子中的维生素C含量均高于普通粉碎法。经过超细粉碎后，果肉中的维生素C含量最高。