The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg g^-1 of extract.

Graphical abstract

中文翻译：

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樟子松叶片中芦丁的高效薄层色谱分析及其药理学和植物化学评价

目前的研究旨在探讨生药学，植物化学和叶芦丁定量海枣通过高性能薄层色谱法（HPTLC）方法。樟子松叶片的各种提取物通过冷浸法在不同溶剂中制备。对提取物进行各种化学测试，以检测各种次生代谢产物，并确定总酚含量（TPC）和总黄酮含量（TFC）的量。通过HPTLC方法对甲醇提取物进行芦丁定量。该方法在线性，准确性，精密度，检出限（LOD），定量限（LOQ）等方面均经过验证。药理学研究主要涵盖叶片的宏观和微观特征，包括粉末显微镜。定性分析显示某些化学成分的存在，例如黄酮类，单宁，有机酸和皂苷苷。樟子松的甲醇提取物显示芦丁的LOD和LOQ分别为125.01 ng / spot和204.921 ng / spot。芦丁的含量为54.6 mg g ^-1提取物。

图形概要