In this study, an in vitro α-chymotrypsin aggregation model was used to demonstrate that certain extracts of commercial coffees effectively inhibit protein aggregation in 55% ethanol at pH 7.0. To detect the anti-amyloidogenic effect of the various coffee extracts, turbidity measurements and Congo red binding assays were performed as well as the determination of the total polyphenol content of the extracts. The greatest fibril formation inhibitory effect was exerted by the Eduscho coffee extract, which contained also the most of the phenolic compounds. The Eduscho coffee extract inhibited the fibrillation of the α-chymotrypsin dose dependently. Coffee extracts are effective anti-aggregation agents, and their beneficial effects strongly correlate with the total phenolic content.

中文翻译：

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咖啡提取物在体外有效抑制乙醇水溶液中α-糜蛋白酶淀粉样纤维的形成

在这项研究中，使用体外α-胰凝乳蛋白酶聚集模型来证明商业咖啡的某些提取物在 pH 7.0 的 55% 乙醇中有效抑制蛋白质聚集。为了检测各种咖啡提取物的抗淀粉样蛋白生成作用，进行了浊度测量和刚果红结合试验，并测定了提取物的总多酚含量。Eduscho咖啡提取物发挥了最大的原纤维形成抑制作用，它也含有最多的酚类化合物。Eduscho 咖啡提取物剂量依赖性地抑制 α-胰凝乳蛋白酶的纤颤。咖啡提取物是有效的抗凝剂，其有益效果与总酚含量密切相关。