Treatments for lesions in central nervous system (CNS) are always faced with challenges due to the anatomical and physiological particularity of the CNS despite the fact that several achievements have been made in early diagnosis and precision medicine to improve the survival and quality of life of patients with brain tumors in recent years. Understanding the complexity as well as role of the microenvironment of brain tumors may suggest a better revealing of the molecular mechanism of brain tumors and new therapeutic directions, which requires an accurate recapitulation of the complex microenvironment of human brain in vitro. Here, a 3D bioprinted in vitro brain matrix-mimetic microenvironment model with hyaluronic acid (HA) and normal glial cells (HEBs) is developed which simulates both mechanical and biological properties of human brain microenvironment in vivo through the investigation of the formulation of bioinks and optimization of printing process and parameters to study the effects of different concentration of gelatin (GA) within the bioink and different printing structures of the scaffold on the performance of the brain matrix-mimetic microenvironment models. The study provides experimental models for the exploration of the multiple factors in the brain microenvironment and scaffolds for GBM invasion study.

中文翻译：

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用于脑微环境模拟的3D生物打印的透明质酸基细胞负载支架

尽管中枢神经系统的解剖学和生理学特殊性，但中枢神经系统（CNS）病变的治疗始终面临挑战，尽管在早期诊断和精密医学方面已经取得了一些成就，以改善患者的生存和生活质量近年来患有脑瘤。了解脑肿瘤微环境的复杂性及其作用可能会更好地揭示脑肿瘤的分子机制和新的治疗方向，这需要在体外​​准确地概括人类脑部复杂的微环境。这里，建立了具有透明质酸（HA）和正常神经胶质细胞（HEBs）的3D生物打印体外脑基质模拟微环境模型，该模型通过研究生物墨水的配方和优化生物模拟来模拟人脑微环境在体内的机械和生物学特性。印刷过程和参数以研究生物墨水中不同浓度的明胶（GA）和支架的不同印刷结构对脑基质模拟微环境模型性能的影响。该研究为探索GBM侵袭研究的大脑微环境和支架中的多种因素提供了实验模型。