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Lentzea indica sp. nov., a novel actinobacteria isolated from Indian Himalayan-soil.
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-07-22 , DOI: 10.1007/s10482-020-01449-8
Pulak Kumar Maiti 1 , Sukhendu Mandal 1
Affiliation  

A novel actinobacterium, strain PSKA42T was isolated from a soil sample of Kashmir Himalaya, India (latitude: 34°-01′ N; longitude: 74°-47′ E; altitude: 5328 ft). The morphological, biochemical and molecular characteristics predicted that PSKA42T belongs to the genera Lentzea. Using the Ezbiocloud server it has also been manifested that the 16S rRNA gene sequence of PSKA42 was 98.4% similar with Lentzea guizhouensis DHSC013T, Lentzea albidocapillata subsp. violacea IMSNU50388T; 98.26% similar with Lentzea aerocolonigenes NRRLB-3298T, Lentzea albidocapillata subsp. albidocapillata NRRLB-24057T and 98.19% similar with Lentzea californiensis DSM43393T. Whole-genome average nucleotide identity values between PSKA42T and L. guizhouensis DHSC013T, L. albidocapillata subsp. violaera IMSNU50388T, L. aerocolonigenes NRRLB-3298T, L. albidocapillata subsp. albidocapillata NRRLB-24057T, L. californiensis DSM43393T, L. flaviverucosa As40578T were 84.94%, 85.8%, 87.15%, 87.71%, 79.29, and 87.22%, respectively, suggesting that PSKA42T represented a new species. In-silco genome-to-genome distances analysis and MLSA with selected housekeeping genes also indicated that the isolate should be assigned to a new species under the genus Lentzea. Analysis of the whole-cell hydrolysate found mannose and ribose as major sugar while cell wall amino acid was identified as meso-diaminopimelic acid. Major fatty acids (> 5%) were observed as 15:0 iso (23.81%), and 16:0 iso (26.11%). PSKA42 contains polyketide synthase type-I genes but unable to show antimicrobial activity in laboratory conditions. The average GC content in its genome is 68.3%. Further, based on physicochemical, chemotaxonomic markers and molecular analysis, PSKA42T can be assigned as novel species of Lentzea, we proposed Lentzea indica sp nov. The type strain is PSKA42T (JCM 33729T, MTCC 12936T, MCC 4127T).



中文翻译:

扁豆印度种。nov。,一种从印度喜马拉雅土壤中分离的新型放线菌。

从印度克什米尔喜马拉雅山的土壤样品中分离出一种新型放线菌菌株PSKA42 T(纬度:N°34°-01';经度:E°74°-47';海拔:5328 ft)。形态,生化和分子特征预示PSKA42 T属于Lentzea属。使用Ezbiocloud服务器也已经体现的是PSKA42的16S rRNA基因序列与类似的98.4%Lentzea贵州苏铁DHSC013牛逼Lentzea albidocapillata亚种。violacea IMSNU50388 Ť ; 98.26%与Lentzea aerocolonigenes NRRLB-3298 TLentzea albidocapillata相似亚种 拟南芥NRRLB- 24057 T和与Lentzea californiensis DSM43393 T的相似度为98.19%。PSKA42 T贵州乳杆菌DHSC013 T白喉乳杆菌亚种之间的全基因组平均核苷酸同一性值。violaera IMSNU50388 T产气链球菌NRRLB-3298 Talbidocapillata亚种。albidocapillata NRRLB-24057 ŤL. californiensis DSM43393 ŤL. flaviverucosa As40578T分别为84.94%,85.8%,87.15%,87.71%,79.29和87.22%,表明PSKA42 T代表了新物种。硅内基因组到基因组的距离分析和带有选定看家基因的MLSA也表明,应该将分离株分配给Lentzea属下的新物种对全细胞水解产物的分析发现甘露糖和核糖为主要糖,而细胞壁氨基酸被鉴定为内消旋二氨基庚二酸。观察到主要脂肪酸(> 5%)为15:0 iso(23.81%)和16:0 iso(26.11%)。PSKA42包含I型聚酮化合物合酶,但在实验室条件下无法显示抗菌活性。其基因组中的平均GC含量为68.3%。此外,基于理化,化学分类标记和分子分析,可以将PSKA42 T指定为Lentzea的新物种,我们提出了Lentzea indica sp nov。类型菌株是PSKA42 T(JCM 33729 T,MTCC 12936 T,MCC 4127 T)。

更新日期:2020-07-24
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