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The gut and feed residue microbiota changing during the rearing of Hermetia illucens larvae.
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-07-07 , DOI: 10.1007/s10482-020-01443-0
Yina Cifuentes 1 , Stefanie P Glaeser 1 , Jacques Mvie 1 , Jens-Ole Bartz 1 , Ariane Müller 2 , Herwig O Gutzeit 2 , Andreas Vilcinskas 3, 4 , Peter Kämpfer 1
Affiliation  

Larvae of Hermetia illucens, commonly known as black soldier fly, efficiently convert organic waste into nutrient-rich supplements for different applications. Here we performed a preliminary experiment to investigate the dynamics of the H. illucens gut microbiota and changes in the composition of the bacterial community in the residue of the larval feed during rearing. We furthermore quantified the presence of antibiotic resistance and disinfectant genes in the gut and feed microbiota during the rearing process to elucidate if rearing leads to a reduction, increase, and/or transfer of resistance genes from the feed to larvae and vice versa. We found that the gut and feed residue bacterial communities were distinct throughout the rearing process. The gut microbiome remained more stable compared to the feed residue microbiome varying in both bacterial abundance and community structure during rearing. Antibiotic-resistance genes were present in both, gut and feed residues, with a significant increase in pupae and residue samples taken at the end of the rearing process. Disinfectant-resistance genes were present in the feed residue and even increased during the rearing process but were not transferred to the gut microbiome. We conclude that H. illucens larvae have a stable gut microbiome that does not change significantly over the course of larval development, whereas bacterial communities in the feed residue are strongly affected by rearing. If the presence of antibiotics and disinfectants during rearing, can promote the spread of antibiotic/disinfectant-resistance genes among feed and larvae needs to be evaluated in further experiments.



中文翻译:

在饲养Hermetia illucens幼虫期间,肠道和饲料残留菌群发生变化。

Hermetia illucens的幼虫通常被称为黑士兵蝇,可以有效地将有机废物转化为营养丰富的补品,以用于不同的应用。在这里,我们进行了初步的实验,以调查illucens的动力学饲养期间幼虫饲料残渣中的肠道菌群和细菌群落组成的变化。我们进一步量化了饲养过程中肠道和饲料微生物区系中抗生素抗性和消毒基因的存在,以阐明饲养是否导致抗性基因从饲料到幼虫的减少,增加和/或转移,反之亦然。我们发现肠道和饲料残留细菌群落在整个饲养过程中是不同的。与饲料残留微生物组相比,肠道微生物组在饲养过程中细菌丰度和群落结构方面均保持稳定。肠道和饲料残留物中均存在抗生素抗性基因,在饲养过程结束时采集的p和残留样品显着增加。饲料残留物中存在抗药性基因,在饲养过程中甚至增加了抗药性基因,但没有转移到肠道微生物组中。我们得出结论llucens illucens幼虫具有稳定的肠道微生物组,在幼虫发育过程中不会发生明显变化,而饲料残渣中的细菌群落则受到饲养的强烈影响。如果在饲养过程中存在抗生素和消毒剂,会促进抗生素/抗药性基因在饲料和幼虫之间的传播,则需要进一步的实验进行评估。

更新日期:2020-07-24
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