Abstract

The tumour suppressor protein p53 regulates various genes involved in cell-cycle arrest, apoptosis and DNA repair in response to cellular stress, and apparently functions as a pioneer transcription factor. The pioneer transcription factors can bind nucleosomal DNA, where many transcription factors are largely restricted. However, the mechanisms by which p53 recognizes the nucleosomal DNA are poorly understood. In the present study, we found that p53 requires linker DNAs for the efficient formation of p53-nucleosome complexes. p53 forms an additional specific complex with the nucleosome, when the p53 binding sequence is located around the entry/exit region of the nucleosomal DNA. We also showed that p53 directly binds to the histone H3-H4 complex via its N-terminal 1–93 amino acid region. These results shed light on the mechanism of nucleosome recognition by p53.

中文翻译：

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接头DNA和组蛋白在p53核小体结合中的作用。

摘要

肿瘤抑制蛋白p53调节细胞周期停滞，凋亡和DNA修复中涉及的各种基因，以响应细胞应激，并显然起先驱转录因子的作用。先锋转录因子可以结合核糖体DNA，而许多转录因子在很大程度上受到限制。但是，对p53识别核小体DNA的机制了解甚少。在本研究中，我们发现p53需要连接子DNA才能有效形成p53-核小体复合物。当p53结合序列位于核小体DNA的进入/退出区域周围时，p53与核小体形成另外的特异性复合物。我们还显示，p53通过其N末端1–93个氨基酸区域直接与组蛋白H3-H4复合物结合。