As one of the important crops in the world, cassava production is seriously threatened by Xanthomonas axonopodis pv. manihotis (Xam) all year round. Calmodulin-binding transcription activators (CAMTAs) play key roles in biotic stress and abiotic stress in plants, however, their roles in cassava remain elusive. In this study, six MeCAMTAs were identified, and MeCAMTA3 with the highest induction upon Xam infection was confirmed as a transcription factor that binds to the vCGCGb motif. MeCAMTA3 negatively regulates plant disease resistance against Xam. On the one hand, MeCAMTA3 negatively regulated endogenous salicylic acid and reactive oxygen species accumulation, pathogenesis-related genes MePRs’ transcripts and callose deposition during cassava-Xam interaction but not under control conditions. On the other hand, RNA sequencing showed extensive transcriptional reprogramming by MeCAMTA3, especially 18 genes with a vCGCGb motif in the promoter region in hormone signaling, antioxidant signaling and other disease resistance signaling. Notably, chromatin immunoprecipitation-polymerase chain reaction showed that eight of these genes might be directly regulated by MeCAMTA3 through transcriptional repression. In summary, MeCAMTA3 negatively regulates plant disease resistance against cassava bacterial blight through modulation of multiple immune responses during cassava-Xam interaction and extensive transcriptional reprogramming.

中文翻译：

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CAMTA3通过调节木薯中的免疫应答和广泛的转录重编程来负调节疾病抵抗力。

作为世界上重要的农作物之一，木薯的生产受到了轴生黄单胞菌的严重威胁。manihotis（Xam）全年。钙调蛋白结合转录激活因子（CAMTA）在植物的生物胁迫和非生物胁迫中起关键作用，但是，它们在木薯中的作用仍然难以捉摸。在这项研究中，确定了六个MeCAMTA，并且证实Xam感染时诱导率最高的MeCAMTA3是与vCGCGb基序结合的转录因子。MeCAMTA3负调节植物对Xam的抗性。一方面，MeCAMTA3对木薯Xam期间内源性水杨酸和活性氧的积累，与病程相关的基因MePRs的转录和ose质沉积具有负调控作用互动，但不在控制条件下。另一方面，RNA测序显示由MeCAMTA3进行的大量转录重编程，特别是在激素信号，抗氧化剂信号和其他抗病信号中的启动子区域具有vCGCGb基序的18个基因。值得注意的是，染色质免疫沉淀-聚合酶链反应显示，其中的八个基因可能通过转录抑制被MeCAMTA3直接调控。总而言之，MeCAMTA3通过在木薯Xam相互作用和广泛的转录重编程过程中调节多种免疫应答来负调节植物对木薯白叶枯病的抗性。